Ace::Graphics::Glyph::graded_segments - The "color-coded segments" glyph


AcePerl documentation Contained in the AcePerl distribution.

Index


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NAME

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Ace::Graphics::Glyph::graded_segments - The "color-coded segments" glyph

SYNOPSIS

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  See L<Ace::Graphics::Glyph::segments>, L<Ace::Graphics::Panel> and L<Ace::Graphics::Glyph>.

DESCRIPTION

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This is identical to the segments glyph, except that the intensity of each segment is proportional to the score of the segment. The maximum score is taken from the configuration variable max_score. If not provided, the maximum-scoring segment will be used.

OPTIONS

In addition to the common options, this glyph recognizes the b<-max_score> argument.

BUGS

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Although descended from the segments glyph, this glyph cannot show the orientation of the segment.

SEE ALSO

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Ace::Sequence, Ace::Sequence::Feature, Ace::Graphics::Panel, Ace::Graphics::Track, Ace::Graphics::Glyph::anchored_arrow, Ace::Graphics::Glyph::arrow, Ace::Graphics::Glyph::box, Ace::Graphics::Glyph::primers, Ace::Graphics::Glyph::segments, Ace::Graphics::Glyph::toomany, Ace::Graphics::Glyph::transcript,

AUTHOR

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Lincoln Stein <lstein@cshl.org>.

Copyright (c) 2001 Cold Spring Harbor Laboratory

This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See DISCLAIMER.txt for disclaimers of warranty.


AcePerl documentation Contained in the AcePerl distribution.

package Ace::Graphics::Glyph::graded_segments;
# package to use for drawing anything that is interrupted
# (has the segment() method) and that has a score associated
# with each segment

use strict;
use vars '@ISA';
use GD;
use Ace::Graphics::Glyph::segments;
@ISA = 'Ace::Graphics::Glyph::segments';

# override draw method
sub draw {
  my $self = shift;

  # bail out if this isn't the right kind of feature
  # handle both das-style and Bio::SeqFeatureI style,
  # which use different names for subparts.
  my @segments;
  my $f = $self->feature;
  if ($f->can('segments')) {
    @segments = $f->segments;

  } elsif ($f->can('sub_SeqFeature')) {
    @segments = $f->sub_SeqFeature;

  } else {
    return $self->SUPER::draw(@_);
  }

  # figure out the colors
  my $max_score = $self->option('max_score');
  unless ($max_score) {
    $max_score = 0;
    foreach (@segments) {
      my $s = eval { $_->score };
      $max_score = $s if $s > $max_score;
    }
  }

  # allocate colors
  my $fill   = $self->fillcolor;
  my %segcolors;
  my ($red,$green,$blue) = $self->factory->rgb($fill);
  foreach (sort {$a->start <=> $b->start} @segments) {
    my $s = eval { $_->score };
    unless (defined $s) {
      $segcolors{$_} = $fill;
      next;
    }
    my($r,$g,$b) = map {(255 - (255-$_) * ($s/$max_score))} ($red,$green,$blue);
    my $idx      = $self->factory->translate($r,$g,$b);
    $segcolors{$_} = $idx;
  }

  # get parameters
  my $gd = shift;
  my ($x1,$y1,$x2,$y2) = $self->calculate_boundaries(@_);
  my ($left,$top) = @_;

  my (@boxes,@skips);

  for (my $i=0; $i < @segments; $i++) {
    my $color = $segcolors{$segments[$i]};

    my ($start,$stop) = ($left + $self->map_pt($segments[$i]->start),
			 $left + $self->map_pt($segments[$i]->end));

    # probably unnecessary, but we do it out of paranaoia
    ($start,$stop) = ($stop,$start) if $start > $stop;

    push @boxes,[$start,$stop,$color];

    if (my $next_segment = $segments[$i+1]) {
      my ($next_start,$next_stop) = ($left + $self->map_pt($next_segment->start),
				     $left + $self->map_pt($next_segment->end));
      # probably unnecessary, but we do it out of paranaoia
      ($next_start,$next_stop) = ($next_stop,$next_start) if $next_start > $next_stop;
      push @skips,[$stop+1,$next_start-1];
    }
  }

  my $fg     = $self->fgcolor;
  my $center = ($y1 + $y2)/2;

  # each skip becomes a simple line
  for my $i (@skips) {
    next unless $i->[1] - $i->[0] >= 1;
    $gd->line($i->[0],$center,$i->[1],$center,$fg);
  }

  # each segment becomes a box
  for my $e (@boxes) {
    my @rect = ($e->[0],$y1,$e->[1],$y2);
    my $color = $e->[2];
    $gd->filledRectangle(@rect,$color);
  }

  # draw label
  $self->draw_label($gd,@_) if $self->option('label');
}
1;

__END__