Bio::NEXUS::Node - Provides functions for manipulating nodes in trees


Bio-NEXUS documentation Contained in the Bio-NEXUS distribution.

Index


Code Index:

NAME

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Bio::NEXUS::Node - Provides functions for manipulating nodes in trees

SYNOPSIS

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new Bio::NEXUS::Node;

DESCRIPTION

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Provides a few useful functions for nodes.

FEEDBACK

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All feedback (bugs, feature enhancements, etc.) are all greatly appreciated. There are no mailing lists at this time for the Bio::NEXUS::Node module, so send all relevant contributions to Dr. Weigang Qiu (weigang@genectr.hunter.cuny.edu).

AUTHORS

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 Weigang Qiu (weigang@genectr.hunter.cuny.edu)
 Eugene Melamud (melamud@carb.nist.gov)
 Chengzhi Liang (liangc@umbi.umd.edu)
 Thomas Hladish (tjhladish at yahoo)

CONTRIBUTORS

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 Peter Yang (pyang@rice.edu)

METHODS

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new

 Title   : new
 Usage   : $node = new Bio::NEXUS::Node();
 Function: Creates a new Bio::NEXUS::Node object
 Returns : Bio::NEXUS::Node object
 Args    : none

clone

 Title   : clone
 Usage   : my $newblock = $block->clone();
 Function: clone a block object (shallow)
 Returns : Block object
 Args    : none

get_seq

 Title   : get_seq
 Usage   : $sequence = $node->get_seq();
 Function: Returns the node's sequence
 Returns : sequence (string)
 Args    : none

set_seq

 Title   : set_seq
 Usage   : $node->set_seq($sequence);
 Function: Sets sequence of the node
 Returns : none
 Args    : sequence (string)

set_parent_node

 Title   : set_parent_node
 Usage   : $node->set_parent_node($parent);
 Function: Sets the parent node of the node
 Returns : none
 Args    : parent node (Bio::NEXUS::Node object)

get_parent

 Title   : get_parent
 Usage   : $parent=$node->get_parent();
 Function: Returns the parent node of the node
 Returns : parent node (Bio::NEXUS::Node object) or undef if nonexistent
 Args    : none

set_length

 Title   : set_length
 Usage   : $node->set_length($length);
 Function: Sets the node's length (meaning the length of the branch leading to the node)
 Returns : none
 Args    : length (number)

get_length

 Title   : length
 Usage   : $length=$node->get_length();
 Function: Returns the node's length
 Returns : length (integer) or undef if nonexistent
 Args    : none

get_total_length

 Title   : get_total_length
 Usage   : $total_length = $node->get_total_length();
 Function: Gets the total branch length of the node and that of all the children (???)
 Returns : total branch length
 Args    : none

set_support_value

 Title   : set_support_value
 Usage   : $node->set_support_value($bootstrap);
 Function: Sets the branch support value associated with this node
 Returns : none
 Args    : bootstrap value (integer)

get_support_value

 Title   : get_support_value
 Usage   : $bootstrap = $node->get_support_value();
 Function: Returns the branch support value associated with this node
 Returns : bootstrap value (integer) or undef if nonexistent
 Args    : none

set_name

 Title   : set_name
 Usage   : $node->set_name($name);
 Function: Sets the node's name
 Returns : none
 Args    : name (string/integer)

get_name

 Title   : get_name
 Usage   : $name = $node->get_name();
 Function: Returns the node's name
 Returns : name (integer/string) or undef if nonexistent
 Args    : none

is_otu

 Title   : is_otu
 Usage   : $node->is_otu();
 Function: Returns 1 if the node is an OTU or 0 if it is not (internal node)
 Returns : 1 or 0
 Args    : none

add_child

 Title   : add_childTU
 Usage   : $node->add_child($node);
 Function: Adds a child to an existing node
 Returns : none
 Args    : child (Bio::NEXUS::Node object)

get_distance

 Title   : get_distance
 Usage   : $distance = $node1->get_distance($node2);
 Function: Calculates tree distance from one node to another (?)
 Returns : distance (floating-point number)
 Args    : target node (Bio::NEXUS::Node objects)

to_string

 Title   : to_string
 Usage   : my $string; $root->tree_string(\$string, 0, $format)
 Function: recursively builds Newick tree string from root to tips 
 Returns : none
 Args    : reference to string, boolean $remove_inode_names flag, string - $format (NHX or STD) 

set_children

 Title   : set_children
 Usage   : $node->set_children($children);
 Function: Sets children
 Returns : $node
 Args    : arrayref of children

get_children

 Title   : get_children
 Usage   : @children = @{ $node->get_children() };
 Function: Retrieves list of children
 Returns : array of children (Bio::NEXUS::Node objects)
 Args    : none

walk

 Title   : walk
 Usage   : @descendents = $node->walk();
 Function: Walks through tree and compiles a "clade list" 
     (including $self and all inodes and otus descended from $self)
 Returns : array of nodes
 Args    : generally, none, though walk() calls itself recurseively with 
     2 arguments: the node list so far, and a counting variable for inode-naming

get_otus

 Title   : get_otus
 Usage   : @listOTU = @{$node->get_otu()}; (?)
 Function: Retrieves list of OTUs
 Returns : reference to array of OTUs (Bio::NEXUS::Node objects)
 Args    : none

printall

 Title   : printall
 Usage   : $tree_as_string = $self->printall(); 
 Function: Gets the node properties as a tabbed string for printing nicely 
           formatted trees (developed by Tom)
 Returns : Formatted string
 Args    : Bio::NEXUS::Node object

find

 Title   : find
 Usage   : $node = $node->find($name);
 Function: Finds the first occurrence of a node called 'name' in the tree
 Returns : Bio::NEXUS::Node object
 Args    : name (string)

prune

 Name    : prune
 Usage   : $node->prune($OTUlist);
 Function: Removes everything from the tree except for OTUs specified in $OTUlist
 Returns : none
 Args    : list of OTUs (string)

equals

 Name    : equals
 Usage   : $node->equals($another_node);
 Function: compare if two nodes (and their subtrees) are equivalent
 Returns : 1 if equal or 0 if not
 Args    : another Node object

get_siblings

 Name    : get_siblings
 Usage   : $node->get_siblings();
 Function: get sibling nodes of this node
 Returns : array ref of sibling nodes
 Args    : none

is_sibling

 Name    : is_sibling
 Usage   : $node1->is_sibling($node2);
 Function: tests whether node1 and node2 are siblings
 Returns : 1 if true, 0 if false
 Args    : second node

adopt

 Title   : adopt
 Usage   : $parent->adopt($child, $overwrite_children);
 Function: make a parent-child relationship between two nodes
 Returns : none
 Args    : the child node, boolean clobber flag

combine

 Title   : combine
 Usage   : my $newblock = $node->combine($child);
 Function: removes a node from the tree, effectively by sliding its only child up the branch to its former position
 Returns : none
 Args    : the child node
 Methods : Combines the child node and the current node by assigning the
           name, bootstrap value, children and other properties of the child.  The branch length
	   of the current node is added to the child node's branch length.

set_depth

 Title   : set_depth
 Usage   : $root->set_depth();
 Function: Determines depth in tree of every node below this one
 Returns : none
 Args    : This node's depth

get_depth

 Title   : get_depth
 Usage   : $depth = $node->get_depth();
 Function: Returns the node's depth (number of 'generations' removed from the root) in tree
 Returns : integer representing node's depth
 Args    : none

find_lengths

 Title   : find_lengths
 Usage   : $cladogram = 1 unless $root->find_lengths();
 Function: Tries to determine if branch lengths are present in the tree
 Returns : 1 if lengths are found, 0 if not
 Args    : none

mrca

 Title     : mrca
 Usage     : $mrca = $otu1-> mrca($otu2, $treename);
 Function  : Finds most recent common ancestor of otu1 and otu2
 Returns   : Node object of most recent common ancestor
 Args      : Nexus object, two otu objects, name of tree to look in

get_mrca_of_otus

 Title     : get_mrca_of_otus
 Usage     : $mrca = $root->get_mrca_of_otus(\@otus);
 Function  : Finds most recent common ancestor of set of OTUs
 Returns   : Node object of most recent common ancestor
 Args      : Nexus object, two otu objects, name of tree to look in

contains_nhx_tag

Title : contains_nhx_tag Usage : $node_obj->_contains_nhx_tag($tag_name) Function: Checks if a given tag exists Returns : 1 if the tax exists, 0 if it doesn't Args : $tag_name - a string representation of a tag

get_nhx_tags

Title : get_nhx_tags Usage : $node_obj->get_nhx_tags(); Function: Reads and returns an array of tags Returns : An array of tags Args : None

get_nhx_values

Title : get_nhx_values Usage : $node_obj->get_nhx_values($tag_name); Function: Returns the list of values associated with a given tag ($tag_name) Returns : Array of values Args : $tag_name - a string representation of the tag

set_nhx_tag

Title : set_nhx_tag Usage : node_obj->set_nhx_tag($tag_name, $tag_reference); Function: Updates the list of values associated with a given tag Returns : Nothing Args : $tag_name - a string, $tag_reference - an array-reference

add_nhx_tag_value

Title : add_nhx_tag_value Usage : $node_obj->add_nhx_tag_value($tag_name, $tag_value); Function: Adds a new tag/value set to the $nhx_obj; Returns : Nothing Args : $tag_name - a string, $tag_reference - an array-reference

delete_nhx_tag

Title : delete_nhx_tag Usage : $node_obj->delete_nhx_tag($tag_name); Function: Removes a given tag (and the associated valus) from the $nhx_obj Returns : Nothing Args : $tag_name - a string representation of the tag

delete_all_nhx_tags

Title : delete_all_nhx_tags Usage : $node_obj->delete_all_nhx_tags(); Function: Removes all tags from $nhx_obj Returns : Nothing Args : None

nhx_command_to_string

Title : nhx_command_to_string Usage : $node_obj->nhx_command_to_string(); Function: As NHX command string Returns : NHX command string Args : None

clone_nhx_command

Title : clone_nhx_command Usage : $some_node_obj->clone_nhx_command($original_node); Function: Copies the data of the NHX command of the $original_node object into the NHX command of the $some_node_obj Returns : Nothing Args : $original_node - Bio::NEXUS::NHXCmd object whose NHX command data will be cloned

check_nhx_tag_value_present

Title : check_nhx_tag_value Usage : $boolean = nhx_obj->check_nhx_tag_value($tag_name, $value); Function: check whether a particular value is present in a tag Returns : 0 or 1 [ true or false] Args : $tag_name - a string, $value - scalar (string or number)

set_nhx_obj

Title : set_nhx_obj Usage : $node->set_nhx_obj($nhx_obj); Function: Sets Bio::NEXUS::NHXCmd object associated with this node Returns : Nothing Args : Reference of the NHXCmd object othing

get_nhx_obj

Title : get_nhx_obj Usage : $nhx_obj = get_nhx_obj(); Function: Returns Bio::NEXUS::NHXCmd object associated with this node Returns : Reference of the NHXCmd object Args : Nothing


Bio-NEXUS documentation Contained in the Bio-NEXUS distribution.
######################################################
# Node.pm
######################################################
# Author:  Weigang Qiu, Eugene Melamud, Chengzhi Liang, Peter Yang, Thomas Hladish, Vivek Gopalan
# $Id: Node.pm,v 1.70 2009/08/13 20:35:55 astoltzfus Exp $

#################### START POD DOCUMENTATION ##################

package Bio::NEXUS::Node;

use strict;
use Bio::NEXUS::Functions;
use Bio::NEXUS::NHXCmd;
use Bio::NEXUS::Util::Exceptions;
use Bio::NEXUS::Util::Logger;
use vars qw($VERSION $AUTOLOAD);
#use Data::Dumper; # XXX this is not used, might as well not import it!
#use Carp; # XXX this is not used, might as well not import it!

use Bio::NEXUS; $VERSION = $Bio::NEXUS::VERSION;
my $logger = Bio::NEXUS::Util::Logger->new();

sub BEGIN {
    eval {
        require warnings;
        1;
        }
        or do {
        no strict 'refs';
        *warnings::import = *warnings::unimport = sub { };
        $INC{'warnings.pm'} = '';
        };
}

sub new {
    my ($class) = @_;
    my $self = { _nhx_obj => undef };
    bless $self, $class;
    return $self;
}

sub clone {
    my ($self) = @_;
    my $class = ref($self);
    my $newnode = bless( { %{$self} }, $class );
    if ( defined $self->{_nhx_obj} ) {
        $newnode->{_nhx_obj} = $self->clone_nhx_command;
    }
    my @children = @{ $newnode->get_children() };
    $newnode->set_children();
    for my $child (@children) {
        my $newchild = $child->clone();
        $newnode->add_child($newchild);
        $newchild->set_parent_node($newnode);
    }
    return $newnode;
}

sub get_seq {
    my ($self) = @_;
    return $self->{'seq'};
}

sub set_seq {
    my ( $self, $seq ) = @_;
    $self->{'seq'} = $seq;
}

sub set_parent_node {
    my ( $self, $parent ) = @_;
    $self->{'parent'} = $parent;
}

sub get_parent {
    if ( defined $_[0]->{'parent'} ) {
        return $_[0]->{'parent'};
    }
    else {
        return undef;
    }
}

sub set_length {
    my ( $self, $length ) = @_;
    $self->{'length'} = $length;
}

sub get_length {
    if ( defined $_[0]->{'length'} ) {
        return $_[0]->{'length'};
    }
    else {
        return undef;
    }
}

sub get_total_length {
    my $self = shift;
    my $len = $self->get_length() || 0;
    for my $child ( @{ $self->get_children() } ) {
        $len += $child->get_total_length();
    }
    return $len;
}

sub set_support_value {
    my ( $self, $bootstrap ) = @_;
    if ( defined $bootstrap and not _is_number($bootstrap) ) {
    	$logger->info("Attempt to set bad branch support value: <$bootstrap> is not a valid number");
    }
    elsif ( not defined $bootstrap ) {
    	$logger->info("Attempt to set undefined branch support value");
    }

    $self->set_nhx_tag( 'B', [$bootstrap] );
}

sub get_support_value {
    my ($self)        = @_;
    my ($support_val) = $self->get_nhx_values('B');
    return $support_val;
}

sub _set_xcoord {
    my ( $self, $xcoord ) = @_;
    $self->{'xcoord'} = $xcoord;
}

sub _get_xcoord {
    if ( defined $_[0]->{'xcoord'} ) {
        return $_[0]->{'xcoord'};
    }
    else {
        return undef;
    }
}

sub _set_ycoord {
    my ( $self, $ycoord ) = @_;
    $self->{'ycoord'} = $ycoord;
}

sub _get_ycoord {
    my $self = shift;
    return $self->{'ycoord'};
}

sub set_name {
    my ( $self, $name ) = @_;
    $self->{'name'} = $name;
}

sub get_name {
    my $self = shift;
    return $self->{'name'};
}

sub is_otu {
    my $self = shift;
    defined $self->{'children'} ? return 0 : return 1;
}

sub add_child {
    my ( $self, $child ) = @_;
    push @{ $self->{'children'} }, $child;
}

sub get_distance {
    my ( $node1, $node2 ) = @_;
    if ( not defined $node2 ) {
    	Bio::NEXUS::Util::Exceptions::BadArgs->throw(
    		'error' => "Missing argument for 'get_distance' method.\n"
    				."The target node is node has to be defined"
    	);
    }
    my $distance = 0;
    if ( $node1 eq $node2 ) {
        return 0;
    }
    my $tmp_node1 = $node1;
    my $tmp_node2 = $node2;

    my %parent1;
    my $common_parent;

    while ( defined $tmp_node1->{'parent'} ) {
        $parent1{$tmp_node1} = 1;
        $tmp_node1 = $tmp_node1->{'parent'};
    }

    #add root node to hash
    $parent1{$tmp_node1} = 1;

    #the following line handles cases where node2 is root
    $common_parent = $tmp_node2;

    while ( not exists $parent1{$tmp_node2} ) {
        if ( defined $tmp_node2->{'parent'} ) {
            $distance += $tmp_node2->get_length();
            $tmp_node2 = $tmp_node2->{'parent'};
        }
        $common_parent = $tmp_node2;
        my $tmp = $common_parent->get_length();
    }

    $tmp_node1 = $node1;    #reset node1
    while ( $tmp_node1 ne $common_parent ) {
        if ( defined $tmp_node1->{'parent'} ) {
            $distance += $tmp_node1->get_length();
            $tmp_node1 = $tmp_node1->{'parent'};
        }
    }
    return $distance;
}

sub to_string {
    my ( $self, $outtree, $remove_inode_names, $out_format ) = @_;

    my $name = $self->get_name();
    $name = _nexus_formatted($name);

    #my $bootstrap = $self->get_support_value();
    my $comment =
        ( $out_format =~ /NHX/i )
        ? $self->nhx_command_to_string
        : $self->get_support_value;
    my $length   = $self->get_length();
    my @children = @{ $self->get_children() };

    if (@children) {    # if $self is an internal node
        $$outtree .= '(';

        for my $child (@children) {
            $child->to_string( $outtree, $remove_inode_names, $out_format );
        }

        $$outtree .= ')';

        if ( defined $name && !$remove_inode_names ) { $$outtree .= $name }
        if ( defined $length )  { $$outtree .= ":$length" }
        if ( defined $comment ) { $$outtree .= "[$comment]" }

        $$outtree .= ',';

    }
    else {    # if $self is a terminal node

        if ( not defined $name ) {
        	Bio::NEXUS::Util::Exceptions::BadArgs->throw(
        		'error' => "OTU found without a name (terminal nodes must be named)"
        	);
        }
        $$outtree .= $name;

        if ( defined $length )  { $$outtree .= ":$length" }
        if ( defined $comment ) { $$outtree .= "[$comment]" }

        $$outtree .= ',';
    }
    $$outtree =~ s/,\)/)/g;
}

sub set_children {
    my ( $self, $children ) = @_;
    $self->{'children'} = $children;
}

sub get_children {
    my $self = shift;
    return $self->{'children'} if ( $self->{'children'} );
    return [];
}

sub walk {
    my ( $self, $nodes, $i ) = @_;

    my $name = $self->get_name();
    # if the node doesn't have a name, name it inode<number>
    if ( !$name ) {
        $self->set_name( 'inode' . $$i++ );
    }

    # if it's not an otu, and the name is a number ('X'), rename it inodeX
    elsif ( !$self->is_otu() && $name =~ /^\d+$/ ) {
        $self->set_name( 'inode' . $name );
    }

    my @children = @{ $self->get_children() };

    # if $self is not an otu,
    if (@children) {
        for my $child (@children) {
            $child->walk( $nodes, $i ) if $child;
        }
    }

    #print scalar @{$nodes}, "\n";;
    push @$nodes, $self;
}

sub get_otus {
    my $self = shift;
    my @otus;
    $self->_walk_otus( \@otus );
    return \@otus;
}

sub _walk_otus {
    my $self  = shift;
    my $nodes = shift;

    my $children = $self->get_children();
    for my $child (@$children) {
        if ( $child->is_otu ) {
            push @$nodes, $child;
        }
        else {
            $child->_walk_otus($nodes) if @$children;
        }
    }
}

sub printall {
    my $self = shift;

    my $children = $self->get_children();
    my $str      = "Name: ";
    $str .= $self->get_name()   if ( $self->get_name() );
    $str .= "   OTU\?: ";
    $str .= $self->is_otu();
    $str .= "    Length: ";
    $str .= $self->get_length() if $self->get_length();

    #$str .= "    bootstrap: ";
    #$str .= $self->get_support_value() if $self->get_support_value();
    $str .= "    Comment: ";
    $str .= $self->nhx_command_to_string() if $self->nhx_command_to_string();
    $str .= "\n";

    #carp($str);
    print $str;

    for my $child (@$children) {
        $child->printall();
    }
}

sub _parse_newick {
    no warnings qw( recursion );
    my ( $self, $words, $pos ) = @_;
    if ( not $words and not @$words ) {
    	Bio::NEXUS::Util::Exceptions::BadArgs->throw(
    		'error' => 'Bio::NEXUS::Node::_parse_newick() called without something to parse'
    	);
    }
    $pos = 0 unless $pos;

    for ( ; $pos < @$words; $pos++ ) {
        my $word = $words->[$pos];

        # For parsing the comments within the NEXUS word.
        $word = $self->_parse_comment($word);
        if ( $word eq '(' ) {
            my $parent_node = $self;

            # start a new clade
            my $new_node = new Bio::NEXUS::Node;
            $parent_node->adopt($new_node);
            $pos = $new_node->_parse_newick( $words, ++$pos );
        }

        # We're starting a sibling of the current node's
        elsif ( $word eq ',' ) {
            my $parent_node = $self->get_parent();
            my $new_node    = new Bio::NEXUS::Node;
            $parent_node->adopt($new_node);
            $pos = $new_node->_parse_newick( $words, ++$pos );
        }

        elsif ( $word eq ')' ) {
            my $parent_node = $self->get_parent();
            $pos = $parent_node->_parse_newick( $words, ++$pos );

            # finish a clade
            last;
        }
        elsif ( $word eq ':' ) {
            $pos = $self->_parse_length( $words, ++$pos );
        }
        else {
            $self->set_name($word);
        }
    }
    return $pos;
}

sub _parse_comment {
    my ( $self, $word ) = @_;
    my $nhx_obj;

    if ( $word =~ s/\[(.*)\]// ) {

        # parse non-empty comment string
        my $comment_str = $1;
        $nhx_obj = new Bio::NEXUS::NHXCmd($comment_str);

        # check if the comment was an NHX command (&&NHX)
        if ( defined $nhx_obj->to_string ) {
            $self->{'is_nhx'} = 1;
        }
        else {
            $self->{'is_nhx'} = 0;
            $nhx_obj->set_tag( 'B', [$comment_str] );
            $self->_parse_support_value($comment_str) if defined $comment_str;

            #$nhx_obj = new Bio::NEXUS::NHXCmd();
            #$nhx_obj->set_tag('B',$support_value);
        }
        $self->{_nhx_obj} = $nhx_obj;
    }
    return $word;
}

sub _parse_length {
    my ( $self, $words, $pos ) = @_;

    my $length;

    # number may have been split up if there were '-' (negative) signs
    until ( !defined $words->[$pos] || $words->[$pos] =~ /^[),]$/ ) {
        $length .= $words->[ $pos++ ];
    }
    --$pos;

    $length = $self->_parse_comment($length);

    # empty branch length definition
    return $pos unless defined $length;

	if ( not _is_number($length) ) {
		Bio::NEXUS::Util::Exceptions::BadNumber->throw(
			'error' => "Bad branch length found in tree string: <$length> is not a valid number"
			
		);
	}

    if ( $length =~ /e/i ) {
        $length = _sci_to_dec($length);
    }
    $self->set_length($length);
    return $pos;
}

sub _parse_support_value {
    my ( $self, $bootstrap ) = @_;

	if ( not _is_number($bootstrap) ) {
		Bio::NEXUS::Util::Exceptions::BadNumber->throw(
			'error' => "Bad branch support value found in tree string: <$bootstrap> is not a valid number"
		);
	}

    $self->set_support_value( _sci_to_dec($bootstrap) )
        if defined _sci_to_dec($bootstrap);
    return $bootstrap;
}

sub find {
    my ( $self, $name ) = @_;
    my $nodename = $self->get_name();
    my $children = $self->get_children();
    return $self if ( $self->get_name() eq $name );
    for my $child (@$children) {
        my $result = $child->find($name);
        return $result if $result;
    }
    return undef;
}

sub prune {
    my ( $self, $OTUlist ) = @_;
    my $name = $self->get_name();
    # the following line is in response to [rt.cpan.org #47707] Bug in Bio::NEXUS::Node.pm
    $name = quotemeta $name; 
    if ( $self->is_otu() ) {
        if ( $OTUlist =~ /\s+$name\s+/ ) {

            # if in the list, keep this OTU
            return "keep";
        }
        else {
            # otherwise, delete it
            return "delete";
        }
    }
    my @children    = @{ $self->get_children() };
    my @newchildren = ();
    for my $child (@children) {
        my $result = $child->prune($OTUlist);
        if ( $result eq "keep" ) {
            push @newchildren, $child;
        }
    }
    $self->{'children'} = \@newchildren;
    if ( $#newchildren == -1 ) {

        # delete the inode because it doesn't have any children
        $self->{'children'} = undef;
        return "delete";
    }
    @children = @{ $self->get_children() };
    if ( $#children == 0 ) {
        my $child     = $children[0];
        my $childname = $children[0]->get_name();

        $self->set_name( $child->get_name() );
        $self->set_seq( $child->get_seq() );
        my $self_length = $self->get_length() || 0;
        $self->set_length( $self_length + $child->get_length() );
        $self->set_support_value( $child->get_support_value() );
        $self->set_nhx_obj( $child->get_nhx_obj()->clone )
            if defined $child->{_nhx_obj};
        $self->_set_xcoord( $child->_get_xcoord() );
        $self->_set_ycoord( $child->_get_ycoord() );
        $self->{'children'} = $child->{'children'};

        if ( $child->is_otu() ) {
            $self->{'children'} = undef;
            undef $self->{'children'};
        }

        # assigning inode $name to child $childname
        return "keep";
    }

    # keeping this inode as is, since it has multiple children
    return "keep";
}

sub equals {
    my ( $self, $other ) = @_;

    # 1 if only one is OTU
    if (   ( $self->is_otu() && !$other->is_otu() )
        || ( !$self->is_otu() && $other->is_otu() ) )
    {

        # not the same
        return 0;
    }

    # 2. both are OTUs
    if ( $self->is_otu() && $other->is_otu() ) {
        if ( $self->_same_attributes($other) ) {

            # ...
            return 1;
        }
        else {
            return 0;
        }
    }

    # 3. neither is OTU
    my @self_children  = @{ $self->get_children() };
    my @other_children = @{ $other->get_children() };

    # compare the attributes of the nodes - see if different
    if ( !$self->_same_attributes($other) ) { return 0; }

    my $num_of_kids = scalar @self_children;

    if ( scalar @self_children != scalar @other_children ) {

        # children are different (their quantity differs)
        return 0;
    }
    else {
        for ( my $self_index = 0; $self_index < $num_of_kids; $self_index++ ) {
            my $found = 'false';

            for (
                my $other_index = $self_index;
                $other_index < $num_of_kids;
                $other_index++
                )
            {

                # the fun part starts here
                # comparing the unsorted arrays of children

                if ( $self_children[$self_index]
                    ->equals( $other_children[$other_index] ) )
                {
                    $found = 'true';

                    # pull out the child that was found and add it to
                    # the front of the array
                    my $temp = $other_children[$other_index];
                    splice( @other_children, $other_index, 1 );
                    unshift( @other_children, $temp );

                    last;
                }
            }

            if ( $found eq 'false' ) {
                return 0;
            }
        }
    }

    return 1;

}

# helper function that compares attributes of two node objects
sub _same_attributes {
    my ( $self, $other ) = @_;

	# mighty one-liner (if the length of one of the nodes is defined, and the length of the other node is not, return false)
	return 0 if (!((defined $self->get_length() && defined $other->get_length())
					||
					(! defined $self->get_length() && ! defined $other->get_length())));
	
	if (defined $self->get_length() && defined $other->get_length()) {
			if ( $self->get_length() != $other->get_length() ) { return 0; }
	}

    # if the nodes are internal, don't check their names...
    # they will most likely differ
    if ( $self->is_otu() && $other->is_otu() ) {
        if ( $self->get_name() ne $other->get_name() ) { return 0; }
    }

    if ( defined $self->get_nhx_obj() && defined $other->get_nhx_obj() ) {
        if ( !$self->get_nhx_obj()->equals( $other->get_nhx_obj ) ) {
            return 0;
        }
    }
    if ( !defined $self->get_nhx_obj() && defined $other->get_nhx_obj() ) {
        return 0;
    }
    if ( defined $self->get_nhx_obj() && !defined $other->get_nhx_obj() ) {
        return 0;
    }

    return 1;
}

sub get_siblings {
    my $self = shift;
    return [] unless defined $self->get_parent;
    my $generation = $self->get_parent()->get_children();
    my $siblings   = [];
    for my $potential_sibling ( @{$generation} ) {
        if ( $potential_sibling ne $self ) {
            push( @$siblings, $potential_sibling );
        }
    }
    return $siblings;
}

sub is_sibling {
    my ( $self, $node2 ) = @_;
    if ( not defined $node2 ) {
    	Bio::NEXUS::Util::Exceptions::BadArgs->throw(
    		'error' => "Missing argument for 'is_sibling' method.\n"
    				. " The node object to test for sibiling has to be given as argument"
    	);
    }
    my $parent1 = $self->get_parent();
    my $parent2 = $node2->get_parent();
    return "1"
        if ( ( defined $parent1 and defined $parent2 )
        and $parent1 eq $parent2 );
    return "0";
}

sub _rearrange {
    my ( $self, $newparent ) = @_;

    # Remove the newparent from this node's children
    $self->set_children( $newparent->get_siblings() );

    # Recursively work up the tree until you get to the node
    my $oldparent = $self->get_parent();
    if ($oldparent) { $oldparent->_rearrange($self); }

    # set new parent as parent, self as child
    $newparent->adopt( $self, 0 );
    $self->set_support_value( $newparent->get_support_value() );
    $self->set_nhx_obj( $newparent->get_nhx_obj()->clone )
        if defined $newparent->{_nhx_obj};
    $self->set_length( $newparent->get_length() );

    return $self;
}

sub adopt {
    my ( $parent, $child, $overwrite_children ) = @_;
    $child->set_parent_node($parent);
    if ($overwrite_children) {
        $parent->set_children( [$child] );
    }
    else {
        $parent->add_child($child);
    }
}

sub combine {
    my ( $self, $child ) = @_;
    $self->set_name( $child->get_name() );
    $self->set_support_value( $child->get_support_value() );
    $self->set_nhx_obj( $child->get_nhx_obj()->clone )
        if defined $child->{_nhx_obj};
    $self->set_length( ( $self->get_length() || 0 ) + $child->get_length() );
    $self->set_children();
    $self->set_children( $child->get_children() )
        if @{ $child->get_children } > 0;
}

# Traverses tree and determines y position of every OTU it finds. If it finds
# an OTU, it adds the current y position to a hash of y coordinates (one key
# for each OTU) and increments the y position.
sub _assign_otu_ycoord {
    my ( $self, $yposref, $spacingref ) = @_;
    return if $self->is_otu();
    for my $child ( @{ $self->get_children() } ) {
        if ( $child->is_otu() ) {
            $child->_set_ycoord($$yposref);
            $$yposref += $$spacingref;
        }
        else {
            $child->_assign_otu_ycoord( $yposref, $spacingref );
        }
    }
}

# Determines position of an internal node (halfway between all its children). Recursive.
sub _assign_inode_ycoord {
    my $self = shift;

    my @tmp;
    for my $child ( @{ $self->get_children() } ) {
        $child->_assign_inode_ycoord() unless ( defined $child->_get_ycoord() );
        push @tmp, $child->_get_ycoord();
    }
    my @sorted = sort { $a <=> $b } @tmp;
    my $high   = pop @sorted;
    my $low    = shift @sorted || $high;
    $self->_set_ycoord( $low + 1 / 2 * ( $high - $low ) );
}

sub set_depth {
    my ( $self, $depth ) = @_;
    $self->{'depth'} = $depth;
    return if $self->is_otu();
    for my $child ( @{ $self->get_children() } ) {
        $child->set_depth( $depth + 1 );
    }
}

sub get_depth {
    my $self = shift;
    return $self->{'depth'};
}

sub find_lengths {
    my $self   = shift;
    my $length = $self->get_length();
    return 1 if ( $length || ( $length = 0 ) );
    for my $child ( @{ $self->get_children() } ) {
        return 1 if $child->find_lengths();
    }
    return 0;
}

sub mrca {
    my ( $otu1, $otu2, $treename ) = @_;
    if ( not $otu1->is_otu and not $otu2->is_otu ) {
    	Bio::NEXUS::Util::Exceptions::ObjectMismatch->throw(
    		'error' => "the mrca method to calculate most recent\n"
    				. "common ancestor can be performed only on\n"
    				. "an OTU node and also requires another OTU\n"
    				. "node (target) as input argument"
    	);
    }

    my $currentnode = $otu1;
    my @ancestors;
    my $mrca;
    until ( $currentnode->get_name() eq 'root' ) {
        $currentnode = $currentnode->get_parent();
        push( @ancestors, $currentnode );
    }
    $currentnode = $otu2;
    until ( $currentnode->get_name() eq 'root' ) {
        $currentnode = $currentnode->get_parent();
        for my $inode (@ancestors) {
            if ( $inode eq $currentnode ) {
                return $inode;
            }
        }
    }
}

sub get_mrca_of_otus {

# Not yet implemented completely. Still in the testing mode -- Vivek Gopalan 10MAR2007.
# Used in assigning meaning inode names to gene tree based on species tree and species names of the OTUs of the inodes.
# Note: Internal nodes can also be given as input instead of the OTU to find the mrca;

    my ($self, $otus, $ancestors ) = @_;
    $ancestors ||= [];
    my @inp_otus = @{$otus};
    my $node_otus =[];
    $self->walk($node_otus);
    my $eq_count = 0 ;
    foreach my $inp_otu (@inp_otus) {
	    if ( grep {$_->get_name eq $inp_otu} @{$node_otus}) {
		    $eq_count++;
		    last if $eq_count == scalar @inp_otus;
	    }
	    #print "$inp_otu $eq_count\n";
    }
    if ($eq_count == scalar @inp_otus) {
	    #print Dumper $ancestors;
	    push @{$ancestors}, $self;
	    foreach my $child ( @{$self->get_children} ) {
		    next if $child->is_otu;
		    $child->get_mrca_of_otus($otus, $ancestors);
	    }
	    #print $self->get_name, "," , $eq_count, ", ", scalar @inp_otus, "\n";
    if (scalar @{$ancestors}) {
	    return $ancestors->[$#{$ancestors}];
    }
    }
    return;

}

sub AUTOLOAD {
	return if $AUTOLOAD =~ /DESTROY$/;
    my $package_name = 'Bio::NEXUS::Node::';

    # The following methods are deprecated and are temporarily supported
    # via a warning and a redirection
    my %synonym_for = (
        "${package_name}depth"       => "${package_name}get_depth",
        "${package_name}boot"        => "${package_name}get_support_value",
        "${package_name}_parse_boot" => "${package_name}_parse_support_value",
        "${package_name}set_boot"    => "${package_name}set_support_value",
        "${package_name}name"        => "${package_name}get_name",
        "${package_name}children"    => "${package_name}get_children",
        "${package_name}length"      => "${package_name}get_length",
        "${package_name}seq"         => "${package_name}get_seq",
        "${package_name}distance"    => "${package_name}get_distance",
        "${package_name}xcoord"      => "${package_name}_get_xcoord",
        "${package_name}ycoord"      => "${package_name}_get_ycoord",
        "${package_name}set_xcoord"  => "${package_name}_set_xcoord",
        "${package_name}set_ycoord"  => "${package_name}_set_ycoord",
        "${package_name}parent_node" => "${package_name}get_parent",
        "${package_name}isOTU"       => "${package_name}is_otu",
        "${package_name}walk_OTUs"   => "${package_name}_walk_otus",
        "${package_name}rearrange"   => "${package_name}_rearrange",
        "${package_name}parse"       => "${package_name}_parse_newick",
    );

    if ( defined $synonym_for{$AUTOLOAD} ) {        
        $logger->warn("$AUTOLOAD() is deprecated; use $synonym_for{$AUTOLOAD}() instead");
        goto &{ $synonym_for{$AUTOLOAD} };
    }
    else {
        Bio::NEXUS::Util::Exceptions::UnknownMethod->throw(
        	'error' => "ERROR: Unknown method $AUTOLOAD called"
        );
    }
    return;
}

################## NHXCmd adapter functions ###############

sub contains_nhx_tag {
    my ( $self, $tag_name ) = @_;
    if ( defined $self->{_nhx_obj} ) {
        return $self->{_nhx_obj}->contains_tag($tag_name);
    }

}    # end of sub

sub get_nhx_tags {
    my ($self) = @_;
    if ( defined $self->{_nhx_obj} ) {
        return $self->{_nhx_obj}->get_tags();
    }
    else {
        return ();
    }
}

sub get_nhx_values {
    my ( $self, $tag_name ) = @_;

    if ( defined $self->{_nhx_obj}
        && $self->{_nhx_obj}->contains_tag($tag_name) )
    {
        return $self->{_nhx_obj}->get_values($tag_name);
    }
    else {
        return ();
    }
}

sub set_nhx_tag {
    my ( $self, $tag_name, $tag_values ) = @_;
	if ( not defined $tag_name || not defined $tag_values ) {
		Bio::NEXUS::Util::Exceptions::BadArgs->throw(
			'error' => "tag_name or tag_values is not defined"
		);
	}
	if ( ref $tag_values ne 'ARRAY' ) {
		Bio::NEXUS::Util::Exceptions::BadArgs->throw(
			'error' => 'tag_values is not an array reference'
		);
	}
    $self->{_nhx_obj} = new Bio::NEXUS::NHXCmd
        unless ( defined $self->{_nhx_obj} );
    $self->{_nhx_obj}->set_tag( $tag_name, $tag_values );

}

sub add_nhx_tag_value {
    my ( $self, $tag_name, $tag_value ) = @_;

    $self->{_nhx_obj} = new Bio::NEXUS::NHXCmd
        unless ( defined $self->{_nhx_obj} );
    return $self->{_nhx_obj}->add_tag_value( $tag_name, $tag_value );

}

sub delete_nhx_tag {
    my ( $self, $tag_name ) = @_;
    if ( defined( $self->{_nhx_obj} ) ) {
        $self->{_nhx_obj}->delete_tag($tag_name);
    }

}

sub delete_all_nhx_tags {
    my ($self) = @_;

    $self->{_nhx_obj}->delete_all_tags() if defined $self->{_nhx_obj};
}

sub nhx_command_to_string {
    my ($self) = @_;
    if ( defined $self->{_nhx_obj} ) {
        return $self->{_nhx_obj}->to_string();
    }
    else {
        return undef;
    }
}

sub clone_nhx_command {
    my ($self) = @_;
    if ( defined $self->{_nhx_obj} ) {
        return $self->{_nhx_obj}->clone();
    }
    else {
        return undef;
    }

}

sub check_nhx_tag_value_present {
    my ( $self, $tag_name, $tag_value ) = @_;


    return $self->{_nhx_obj}->check_tag_value_present( $tag_name, $tag_value )
        if defined $self->{_nhx_obj};
}

sub set_nhx_obj {
    my ( $self, $nhx_obj ) = @_;
    $self->{_nhx_obj} = $nhx_obj;
}

sub get_nhx_obj {
    my ($self) = @_;
    return $self->{_nhx_obj};
}

1;