Bio::Phylo::Forest::Node - Node in a phylogenetic tree


Bio-Phylo documentation Contained in the Bio-Phylo distribution.

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NAME

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Bio::Phylo::Forest::Node - Node in a phylogenetic tree

SYNOPSIS

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 # some way to get nodes:
 use Bio::Phylo::IO;
 my $string = '((A,B),C);';
 my $forest = Bio::Phylo::IO->parse(
    -format => 'newick',
    -string => $string
 );

 # prints 'Bio::Phylo::Forest'
 print ref $forest;

 foreach my $tree ( @{ $forest->get_entities } ) {

    # prints 'Bio::Phylo::Forest::Tree'
    print ref $tree;

    foreach my $node ( @{ $tree->get_entities } ) {

       # prints 'Bio::Phylo::Forest::Node'
       print ref $node;

       # node has a parent, i.e. is not root
       if ( $node->get_parent ) {
          $node->set_branch_length(1);
       }

       # node is root
       else {
          $node->set_branch_length(0);
       }
    }
 }

DESCRIPTION

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This module defines a node object and its methods. The node is fairly syntactically rich in terms of navigation, and additional getters are provided to further ease navigation from node to node. Typical first daughter -> next sister traversal and recursion is possible, but there are also shrinkwrapped methods that return for example all terminal descendants of the focal node, or all internals, etc.

Node objects are inserted into tree objects, although technically the tree object is only a container holding all the nodes together. Unless there are orphans all nodes can be reached without recourse to the tree object.

METHODS

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CONSTRUCTOR

new()

Node constructor.

 Type    : Constructor
 Title   : new
 Usage   : my $node = Bio::Phylo::Forest::Node->new;
 Function: Instantiates a Bio::Phylo::Forest::Node object
 Returns : Bio::Phylo::Forest::Node
 Args    : All optional:
           -parent          => $parent,
           -taxon           => $taxon,
           -branch_length   => 0.423e+2,
           -first_daughter  => $f_daughter,
           -last_daughter   => $l_daughter,
           -next_sister     => $n_sister,
           -previous_sister => $p_sister,
           -name            => 'node_name',
           -desc            => 'this is a node',
           -score           => 0.98,
           -generic         => {
                -posterior => 0.98,
                -bootstrap => 0.80
           }

new_from_bioperl()

Node constructor from bioperl Bio::Tree::NodeI argument.

 Type    : Constructor
 Title   : new_from_bioperl
 Usage   : my $node =
           Bio::Phylo::Forest::Node->new_from_bioperl(
               $bpnode
           );
 Function: Instantiates a Bio::Phylo::Forest::Node object
           from a bioperl node object.
 Returns : Bio::Phylo::Forest::Node
 Args    : An objects that implements Bio::Tree::NodeI
 Notes   : The following BioPerl properties are copied:
           BioPerl output:        Bio::Phylo output:
           ------------------------------------------------
           id                     get_name
           branch_length          get_branch_length
           description            get_desc
           bootstrap              get_generic('bootstrap')

           In addition all BioPerl tags and values are copied
           to set_generic( 'tag' => 'value' );

MUTATORS

prune_child()

Removes argument child node (and its descendants) from invocants children.

 Type    : Mutator
 Title   : prune_child
 Usage   : $parent->prune_child($child);
 Function: Removes $child (and its descendants) from $parent's children
 Returns : Modified object.
 Args    : A valid argument is Bio::Phylo::Forest::Node object.

collapse()

Collapse node.

 Type    : Mutator
 Title   : collapse
 Usage   : $node->collapse;
 Function: Attaches invocant's children to invocant's parent.
 Returns : Modified object.
 Args    : NONE
 Comments: If defined, adds invocant's branch 
           length to that of its children. If
           $node is in a tree, removes itself
           from that tree.

set_parent()

Sets argument as invocant's parent.

 Type    : Mutator
 Title   : set_parent
 Usage   : $node->set_parent($parent);
 Function: Assigns a node's parent.
 Returns : Modified object.
 Args    : If no argument is given, the current
           parent is set to undefined. A valid
           argument is Bio::Phylo::Forest::Node
           object.

set_first_daughter()

Sets argument as invocant's first daughter.

 Type    : Mutator
 Title   : set_first_daughter
 Usage   : $node->set_first_daughter($f_daughter);
 Function: Assigns a node's leftmost daughter.
 Returns : Modified object.
 Args    : Undefines the first daughter if no
           argument given. A valid argument is
           a Bio::Phylo::Forest::Node object.

set_last_daughter()

Sets argument as invocant's last daughter.

 Type    : Mutator
 Title   : set_last_daughter
 Usage   : $node->set_last_daughter($l_daughter);
 Function: Assigns a node's rightmost daughter.
 Returns : Modified object.
 Args    : A valid argument consists of a
           Bio::Phylo::Forest::Node object. If
           no argument is given, the value is
           set to undefined.

set_previous_sister()

Sets argument as invocant's previous sister.

 Type    : Mutator
 Title   : set_previous_sister
 Usage   : $node->set_previous_sister($p_sister);
 Function: Assigns a node's previous sister (to the left).
 Returns : Modified object.
 Args    : A valid argument consists of
           a Bio::Phylo::Forest::Node object.
           If no argument is given, the value
           is set to undefined.

set_next_sister()

Sets argument as invocant's next sister.

 Type    : Mutator
 Title   : set_next_sister
 Usage   : $node->set_next_sister($n_sister);
 Function: Assigns or retrieves a node's
           next sister (to the right).
 Returns : Modified object.
 Args    : A valid argument consists of a
           Bio::Phylo::Forest::Node object.
           If no argument is given, the
           value is set to undefined.

set_child()

Sets argument as invocant's child.

 Type    : Mutator
 Title   : set_child
 Usage   : $node->set_child($child);
 Function: Assigns a new child to $node
 Returns : Modified object.
 Args    : A valid argument consists of a
           Bio::Phylo::Forest::Node object.

set_branch_length()

Sets argument as invocant's branch length.

 Type    : Mutator
 Title   : set_branch_length
 Usage   : $node->set_branch_length(0.423e+2);
 Function: Assigns a node's branch length.
 Returns : Modified object.
 Args    : If no argument is given, the
           current branch length is set
           to undefined. A valid argument
           is a number in any of Perl's formats.

set_node_below()

Sets new (unbranched) node below invocant.

 Type    : Mutator
 Title   : set_node_below
 Usage   : my $new_node = $node->set_node_below;
 Function: Creates a new node below $node
 Returns : New node if tree was modified, undef otherwise
 Args    : NONE

set_root_below()

Reroots below invocant.

 Type    : Mutator
 Title   : set_root_below
 Usage   : $node->set_root_below;
 Function: Creates a new tree root below $node
 Returns : New root if tree was modified, undef otherwise
 Args    : NONE
 Comments: Implementation incomplete: returns spurious 
           results when $node is grandchild of current root.

set_tree()

Sets what tree invocant belongs to

 Type    : Mutator
 Title   : set_tree
 Usage   : $node->set_tree($tree);
 Function: Sets what tree invocant belongs to
 Returns : Invocant
 Args    : Bio::Phylo::Forest::Tree
 Comments: This method is called automatically 
           when inserting or deleting nodes in
           trees.

ACCESSORS

get_parent()

Gets invocant's parent.

 Type    : Accessor
 Title   : get_parent
 Usage   : my $parent = $node->get_parent;
 Function: Retrieves a node's parent.
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_first_daughter()

Gets invocant's first daughter.

 Type    : Accessor
 Title   : get_first_daughter
 Usage   : my $f_daughter = $node->get_first_daughter;
 Function: Retrieves a node's leftmost daughter.
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_last_daughter()

Gets invocant's last daughter.

 Type    : Accessor
 Title   : get_last_daughter
 Usage   : my $l_daughter = $node->get_last_daughter;
 Function: Retrieves a node's rightmost daughter.
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_previous_sister()

Gets invocant's previous sister.

 Type    : Accessor
 Title   : get_previous_sister
 Usage   : my $p_sister = $node->get_previous_sister;
 Function: Retrieves a node's previous sister (to the left).
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_next_sister()

Gets invocant's next sister.

 Type    : Accessor
 Title   : get_next_sister
 Usage   : my $n_sister = $node->get_next_sister;
 Function: Retrieves a node's next sister (to the right).
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_branch_length()

Gets invocant's branch length.

 Type    : Accessor
 Title   : get_branch_length
 Usage   : my $branch_length = $node->get_branch_length;
 Function: Retrieves a node's branch length.
 Returns : FLOAT
 Args    : NONE
 Comments: Test for "defined($node->get_branch_length)"
           for zero-length (but defined) branches. Testing
           "if ( $node->get_branch_length ) { ... }"
           yields false for zero-but-defined branches!

get_ancestors()

Gets invocant's ancestors.

 Type    : Query
 Title   : get_ancestors
 Usage   : my @ancestors = @{ $node->get_ancestors };
 Function: Returns an array reference of ancestral nodes,
           ordered from young to old (i.e. $ancestors[-1] is root).
 Returns : Array reference of Bio::Phylo::Forest::Node
           objects.
 Args    : NONE

get_root()

Gets root relative to the invocant, i.e. by walking up the path of ancestors

 Type    : Query
 Title   : get_root
 Usage   : my $root = $node->get_root;
 Function: Gets root relative to the invocant
 Returns : Bio::Phylo::Forest::Node           
 Args    : NONE

get_farthest_node()

Gets node farthest away from the invocant. By default this is nodal distance, but when supplied an optional true argument it is based on patristic distance instead.

 Type    : Query
 Title   : get_farthest_node
 Usage   : my $farthest = $node->get_farthest_node;
 Function: Gets node farthest away from the invocant.
 Returns : Bio::Phylo::Forest::Node           
 Args    : Optional, TRUE value to use patristic instead of nodal distance

get_sisters()

Gets invocant's sisters.

 Type    : Query
 Title   : get_sisters
 Usage   : my @sisters = @{ $node->get_sisters };
 Function: Returns an array reference of sisters,
           ordered from left to right.
 Returns : Array reference of
           Bio::Phylo::Forest::Node objects.
 Args    : NONE

get_children()

Gets invocant's immediate children.

 Type    : Query
 Title   : get_children
 Usage   : my @children = @{ $node->get_children };
 Function: Returns an array reference of immediate
           descendants, ordered from left to right.
 Returns : Array reference of
           Bio::Phylo::Forest::Node objects.
 Args    : NONE

get_child()

Gets invocant's i'th child.

 Type    : Query
 Title   : get_child
 Usage   : my $child = $node->get_child($i);
 Function: Returns the child at index $i
 Returns : A Bio::Phylo::Forest::Node object.
 Args    : An index (integer) $i
 Comments: if no index is specified, first
           child is returned

get_descendants()

Gets invocant's descendants.

 Type    : Query
 Title   : get_descendants
 Usage   : my @descendants = @{ $node->get_descendants };
 Function: Returns an array reference of
           descendants, recursively ordered
           breadth first.
 Returns : Array reference of
           Bio::Phylo::Forest::Node objects.
 Args    : none.

get_terminals()

Gets invocant's terminal descendants.

 Type    : Query
 Title   : get_terminals
 Usage   : my @terminals = @{ $node->get_terminals };
 Function: Returns an array reference
           of terminal descendants.
 Returns : Array reference of
           Bio::Phylo::Forest::Node objects.
 Args    : NONE

get_internals()

Gets invocant's internal descendants.

 Type    : Query
 Title   : get_internals
 Usage   : my @internals = @{ $node->get_internals };
 Function: Returns an array reference
           of internal descendants.
 Returns : Array reference of
           Bio::Phylo::Forest::Node objects.
 Args    : NONE

get_mrca()

Gets invocant's most recent common ancestor shared with argument.

 Type    : Query
 Title   : get_mrca
 Usage   : my $mrca = $node->get_mrca($other_node);
 Function: Returns the most recent common ancestor
           of $node and $other_node.
 Returns : Bio::Phylo::Forest::Node
 Args    : A Bio::Phylo::Forest::Node
           object in the same tree.

get_leftmost_terminal()

Gets invocant's leftmost terminal descendant.

 Type    : Query
 Title   : get_leftmost_terminal
 Usage   : my $leftmost_terminal =
           $node->get_leftmost_terminal;
 Function: Returns the leftmost
           terminal descendant of $node.
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_rightmost_terminal()

Gets invocant's rightmost terminal descendant

 Type    : Query
 Title   : get_rightmost_terminal
 Usage   : my $rightmost_terminal =
           $node->get_rightmost_terminal;
 Function: Returns the rightmost
           terminal descendant of $node.
 Returns : Bio::Phylo::Forest::Node
 Args    : NONE

get_tree()

Returns the tree invocant belongs to

 Type    : Query
 Title   : get_tree
 Usage   : my $tree = $node->get_tree;
 Function: Returns the tree $node belongs to
 Returns : Bio::Phylo::Forest::Tree
 Args    : NONE

get_subtree()

Returns the tree subtended by the invocant

 Type    : Query
 Title   : get_subtree
 Usage   : my $tree = $node->get_subtree;
 Function: Returns the tree subtended by the invocant
 Returns : Bio::Phylo::Forest::Tree
 Args    : NONE

TESTS

is_terminal()

Tests if invocant is a terminal node.

 Type    : Test
 Title   : is_terminal
 Usage   : if ( $node->is_terminal ) {
              # do something
           }
 Function: Returns true if node has
           no children (i.e. is terminal).
 Returns : BOOLEAN
 Args    : NONE

is_internal()

Tests if invocant is an internal node.

 Type    : Test
 Title   : is_internal
 Usage   : if ( $node->is_internal ) {
              # do something
           }
 Function: Returns true if node
           has children (i.e. is internal).
 Returns : BOOLEAN
 Args    : NONE

is_preterminal()

Tests if all direct descendents are terminal

 Type    : Test
 Title   : is_preterminal
 Usage   : if ( $node->is_preterminal ) {
              # do something
           }
 Function: Returns true if all direct descendents are terminal
 Returns : BOOLEAN
 Args    : NONE

is_first()

Tests if invocant is first sibling in left-to-right order.

 Type    : Test
 Title   : is_first
 Usage   : if ( $node->is_first ) {
              # do something
           }
 Function: Returns true if first sibling 
           in left-to-right order.
 Returns : BOOLEAN
 Args    : NONE

is_last()

Tests if invocant is last sibling in left-to-right order.

 Type    : Test
 Title   : is_last
 Usage   : if ( $node->is_last ) {
              # do something
           }
 Function: Returns true if last sibling 
           in left-to-right order.
 Returns : BOOLEAN
 Args    : NONE

is_root()

Tests if invocant is a root.

 Type    : Test
 Title   : is_root
 Usage   : if ( $node->is_root ) {
              # do something
           }
 Function: Returns true if node is a root       
 Returns : BOOLEAN
 Args    : NONE

is_descendant_of()

Tests if invocant is descendant of argument.

 Type    : Test
 Title   : is_descendant_of
 Usage   : if ( $node->is_descendant_of($grandparent) ) {
              # do something
           }
 Function: Returns true if the node is
           a descendant of the argument.
 Returns : BOOLEAN
 Args    : putative ancestor - a
           Bio::Phylo::Forest::Node object.

is_ancestor_of()

Tests if invocant is ancestor of argument.

 Type    : Test
 Title   : is_ancestor_of
 Usage   : if ( $node->is_ancestor_of($grandchild) ) {
              # do something
           }
 Function: Returns true if the node
           is an ancestor of the argument.
 Returns : BOOLEAN
 Args    : putative descendant - a
           Bio::Phylo::Forest::Node object.

is_sister_of()

Tests if invocant is sister of argument.

 Type    : Test
 Title   : is_sister_of
 Usage   : if ( $node->is_sister_of($sister) ) {
              # do something
           }
 Function: Returns true if the node is
           a sister of the argument.
 Returns : BOOLEAN
 Args    : putative sister - a
           Bio::Phylo::Forest::Node object.

is_child_of()

Tests if invocant is child of argument.

 Type    : Test
 Title   : is_child_of
 Usage   : if ( $node->is_child_of($parent) ) {
              # do something
           }
 Function: Returns true if the node is
           a child of the argument.
 Returns : BOOLEAN
 Args    : putative parent - a
           Bio::Phylo::Forest::Node object.

is_outgroup_of()

Test if invocant is outgroup of argument nodes.

 Type    : Test
 Title   : is_outgroup_of
 Usage   : if ( $node->is_outgroup_of(\@ingroup) ) {
              # do something
           }
 Function: Tests whether the set of
           \@ingroup is monophyletic
           with respect to the $node.
 Returns : BOOLEAN
 Args    : A reference to an array of
           Bio::Phylo::Forest::Node objects;
 Comments: This method is essentially the same as
           &Bio::Phylo::Forest::Tree::is_monophyletic.

can_contain()

Test if argument(s) can be a child/children of invocant.

 Type    : Test
 Title   : can_contain
 Usage   : if ( $parent->can_contain(@children) ) {
              # do something
           }
 Function: Test if arguments can be children of invocant.
 Returns : BOOLEAN
 Args    : An array of Bio::Phylo::Forest::Node objects;
 Comments: This method is an override of 
           Bio::Phylo::Listable::can_contain. Since node
           objects hold a list of their children, they
           inherit from the listable class and so they
           need to be able to validate the contents
           of that list before they are inserted.

CALCULATIONS

calc_path_to_root()

Calculates path to root.

 Type    : Calculation
 Title   : calc_path_to_root
 Usage   : my $path_to_root =
           $node->calc_path_to_root;
 Function: Returns the sum of branch
           lengths from $node to the root.
 Returns : FLOAT
 Args    : NONE

calc_nodes_to_root()

Calculates number of nodes to root.

 Type    : Calculation
 Title   : calc_nodes_to_root
 Usage   : my $nodes_to_root =
           $node->calc_nodes_to_root;
 Function: Returns the number of nodes
           from $node to the root.
 Returns : INT
 Args    : NONE

calc_max_nodes_to_tips()

Calculates maximum number of nodes to tips.

 Type    : Calculation
 Title   : calc_max_nodes_to_tips
 Usage   : my $max_nodes_to_tips =
           $node->calc_max_nodes_to_tips;
 Function: Returns the maximum number
           of nodes from $node to tips.
 Returns : INT
 Args    : NONE

calc_min_nodes_to_tips()

Calculates minimum number of nodes to tips.

 Type    : Calculation
 Title   : calc_min_nodes_to_tips
 Usage   : my $min_nodes_to_tips =
           $node->calc_min_nodes_to_tips;
 Function: Returns the minimum number of
           nodes from $node to tips.
 Returns : INT
 Args    : NONE

calc_max_path_to_tips()

Calculates longest path to tips.

 Type    : Calculation
 Title   : calc_max_path_to_tips
 Usage   : my $max_path_to_tips =
           $node->calc_max_path_to_tips;
 Function: Returns the path length from
           $node to the tallest tip.
 Returns : FLOAT
 Args    : NONE

calc_min_path_to_tips()

Calculates shortest path to tips.

 Type    : Calculation
 Title   : calc_min_path_to_tips
 Usage   : my $min_path_to_tips =
           $node->calc_min_path_to_tips;
 Function: Returns the path length from
           $node to the shortest tip.
 Returns : FLOAT
 Args    : NONE

calc_patristic_distance()

Calculates patristic distance between invocant and argument.

 Type    : Calculation
 Title   : calc_patristic_distance
 Usage   : my $patristic_distance =
           $node->calc_patristic_distance($other_node);
 Function: Returns the patristic distance
           between $node and $other_node.
 Returns : FLOAT
 Args    : Bio::Phylo::Forest::Node

calc_nodal_distance()

Calculates node distance between invocant and argument.

 Type    : Calculation
 Title   : calc_nodal_distance
 Usage   : my $nodal_distance =
           $node->calc_nodal_distance($other_node);
 Function: Returns the number of nodes
           between $node and $other_node.
 Returns : INT
 Args    : Bio::Phylo::Forest::Node

VISITOR METHODS

The methods below are similar in spirit to those by the same name in Bio::Phylo::Forest::Tree, except those in the tree class operate from the tree root, and those in this node class operate on an invocant node, and so these process a subtree.

visit_depth_first()

Visits nodes depth first

 Type    : Visitor method
 Title   : visit_depth_first
 Usage   : $tree->visit_depth_first( -pre => sub{ ... }, -post => sub { ... } );
 Function: Visits nodes in a depth first traversal, executes subs
 Returns : $tree
 Args    : Optional:
            # first event handler, is executed when node is reached in recursion
            -pre            => sub { print "pre: ",            shift->get_name, "\n" },

            # is executed if node has a daughter, but before that daughter is processed
            -pre_daughter   => sub { print "pre_daughter: ",   shift->get_name, "\n" },

            # is executed if node has a daughter, after daughter has been processed 
            -post_daughter  => sub { print "post_daughter: ",  shift->get_name, "\n" },

            # is executed if node has no daughter
            -no_daughter    => sub { print "no_daughter: ",    shift->get_name, "\n" },                         

            # is executed whether or not node has sisters, if it does have sisters
            # they're processed first   
            -in             => sub { print "in: ",             shift->get_name, "\n" },

            # is executed if node has a sister, before sister is processed
            -pre_sister     => sub { print "pre_sister: ",     shift->get_name, "\n" }, 

            # is executed if node has a sister, after sister is processed
            -post_sister    => sub { print "post_sister: ",    shift->get_name, "\n" },         

            # is executed if node has no sister
            -no_sister      => sub { print "no_sister: ",      shift->get_name, "\n" }, 

            # is executed last          
            -post           => sub { print "post: ",           shift->get_name, "\n" },

            # specifies traversal order, default 'ltr' means first_daugher -> next_sister
            # traversal, alternate value 'rtl' means last_daughter -> previous_sister traversal
            -order          => 'ltr', # ltr = left-to-right, 'rtl' = right-to-left

            # passes sister node as second argument to pre_sister and post_sister subs,
            # and daughter node as second argument to pre_daughter and post_daughter subs
            -with_relatives => 1 # or any other true value
 Comments: 

visit_breadth_first()

Visits nodes breadth first

 Type    : Visitor method
 Title   : visit_breadth_first
 Usage   : $tree->visit_breadth_first( -pre => sub{ ... }, -post => sub { ... } );
 Function: Visits nodes in a breadth first traversal, executes handlers
 Returns : $tree
 Args    : Optional handlers in the order in which they would be executed on an internal node:

            # first event handler, is executed when node is reached in recursion
            -pre            => sub { print "pre: ",            shift->get_name, "\n" },

            # is executed if node has a sister, before sister is processed
            -pre_sister     => sub { print "pre_sister: ",     shift->get_name, "\n" }, 

            # is executed if node has a sister, after sister is processed
            -post_sister    => sub { print "post_sister: ",    shift->get_name, "\n" },         

            # is executed if node has no sister
            -no_sister      => sub { print "no_sister: ",      shift->get_name, "\n" },             

            # is executed whether or not node has sisters, if it does have sisters
            # they're processed first   
            -in             => sub { print "in: ",             shift->get_name, "\n" },         

            # is executed if node has a daughter, but before that daughter is processed
            -pre_daughter   => sub { print "pre_daughter: ",   shift->get_name, "\n" },

            # is executed if node has a daughter, after daughter has been processed 
            -post_daughter  => sub { print "post_daughter: ",  shift->get_name, "\n" },

            # is executed if node has no daughter
            -no_daughter    => sub { print "no_daughter: ",    shift->get_name, "\n" },                         

            # is executed last          
            -post           => sub { print "post: ",           shift->get_name, "\n" },

            # specifies traversal order, default 'ltr' means first_daugher -> next_sister
            # traversal, alternate value 'rtl' means last_daughter -> previous_sister traversal
            -order          => 'ltr', # ltr = left-to-right, 'rtl' = right-to-left
 Comments: 

visit_level_order()

Visits nodes in a level order traversal.

 Type    : Visitor method
 Title   : visit_level_order
 Usage   : $tree->visit_level_order( sub{...} );
 Function: Visits nodes in a level order traversal, executes sub
 Returns : $tree
 Args    : A subroutine reference that operates on visited nodes.
 Comments:

UTILITY METHODS

clone()

Clones invocant.

 Type    : Utility method
 Title   : clone
 Usage   : my $clone = $object->clone;
 Function: Creates a copy of the invocant object.
 Returns : A copy of the invocant.
 Args    : Optional: a hash of code references to 
           override reflection-based getter/setter copying

           my $clone = $object->clone(  
               'set_forest' => sub {
                   my ( $self, $clone ) = @_;
                   for my $forest ( @{ $self->get_forests } ) {
                       $clone->set_forest( $forest );
                   }
               },
               'set_matrix' => sub {
                   my ( $self, $clone ) = @_;
                   for my $matrix ( @{ $self->get_matrices } ) {
                       $clone->set_matrix( $matrix );
                   }
           );

 Comments: Cloning is currently experimental, use with caution.
           It works on the assumption that the output of get_foo
           called on the invocant is to be provided as argument
           to set_foo on the clone - such as 
           $clone->set_name( $self->get_name ). Sometimes this 
           doesn't work, for example where this symmetry doesn't
           exist, or where the return value of get_foo isn't valid
           input for set_foo. If such a copy fails, a warning is 
           emitted. To make sure all relevant attributes are copied
           into the clone, additional code references can be 
           provided, as in the example above. Typically, this is
           done by overrides of this method in child classes.

SERIALIZERS

to_xml()

Serializes invocant to xml.

 Type    : Serializer
 Title   : to_xml
 Usage   : my $xml = $obj->to_xml;
 Function: Turns the invocant object (and its descendants )into an XML string.
 Returns : SCALAR
 Args    : NONE

to_newick()

Serializes subtree subtended by invocant to newick string.

 Type    : Serializer
 Title   : to_newick
 Usage   : my $newick = $obj->to_newick;
 Function: Turns the invocant object into a newick string.
 Returns : SCALAR
 Args    : takes same arguments as Bio::Phylo::Unparsers::Newick
 Comments: takes same arguments as Bio::Phylo::Unparsers::Newick

to_dom()
 Type    : Serializer
 Title   : to_dom
 Usage   : $node->to_dom($dom)
 Function: Generates an array of DOM elements from the invocant's
           descendants
 Returns : an array of Element objects
 Args    : DOM factory object

SEE ALSO

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Bio::Phylo::Taxa::TaxonLinker

This object inherits from Bio::Phylo::Taxa::TaxonLinker, so methods defined there are also applicable here.

Bio::Phylo::Listable

This object inherits from Bio::Phylo::Listable, so methods defined there are also applicable here.

Bio::Phylo::Manual

Also see the manual: Bio::Phylo::Manual and http://rutgervos.blogspot.com.

CITATION

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If you use Bio::Phylo in published research, please cite it:

Rutger A Vos, Jason Caravas, Klaas Hartmann, Mark A Jensen and Chase Miller, 2011. Bio::Phylo - phyloinformatic analysis using Perl. BMC Bioinformatics 12:63. http://dx.doi.org/10.1186/1471-2105-12-63

REVISION

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 $Id: Node.pm 1660 2011-04-02 18:29:40Z rvos $


Bio-Phylo documentation Contained in the Bio-Phylo distribution.
# $Id: Node.pm 1660 2011-04-02 18:29:40Z rvos $
package Bio::Phylo::Forest::Node;
use strict;
use base qw'Bio::Phylo::Taxa::TaxonLinker Bio::Phylo::Listable';
use Bio::Phylo::Util::OptionalInterface 'Bio::Tree::NodeI';
use Bio::Phylo::Util::CONSTANT qw':objecttypes /looks_like/';
use Bio::Phylo::Util::Exceptions 'throw';
use Bio::Phylo::NeXML::Writable;
use Bio::Phylo::Factory;
use Scalar::Util 'weaken';
no warnings 'recursion';
my $LOADED_WRAPPERS = 0;
{
    my $fac = Bio::Phylo::Factory->new;

    # logger singleton
    my $logger = __PACKAGE__->get_logger;

    # store type constant
    my ( $TYPE_CONSTANT, $CONTAINER_CONSTANT ) = ( _NODE_, _TREE_ );

    # @fields array necessary for object destruction
    my @fields = \( my ( %branch_length, %parent, %tree ) );

    sub new {

        # could be child class
        my $class = shift;

        # notify user
        $logger->info("constructor called for '$class'");

        # process bioperl args
        my %args = looks_like_hash @_;
        if ( exists $args{'-leaf'} ) {
            delete $args{'-leaf'};
        }
        if ( exists $args{'-id'} ) {
            my $name = $args{'-id'};
            delete $args{'-id'};
            $args{'-name'} = $name;
        }
        if ( exists $args{'-nhx'} ) {
            my $hash = $args{'-nhx'};
            delete $args{'-nhx'};
            $args{'-generic'} = $hash;
        }

        # 		if ( not exists $args{'-tag'} ) {
        # 			$args{'-tag'} = __PACKAGE__->_tag;
        # 		}
        # go up inheritance tree, eventually get an ID
        my $self = $class->SUPER::new(%args);
        if ( not $LOADED_WRAPPERS ) {
            eval do { local $/; <DATA> };
            $LOADED_WRAPPERS++;
        }
        return $self;
    }
    my $set_raw_parent = sub {
        my ( $self, $parent ) = @_;
        my $id = $self->get_id;
        $parent{$id} = $parent;    # XXX here we modify parent
        weaken $parent{$id} if $parent;
    };
    my $get_parent = sub {
        my $self = shift;
        return $parent{ $self->get_id };
    };
    my $get_children = sub { shift->get_entities };
    my $get_branch_length = sub {
        my $self = shift;
        return $branch_length{ $self->get_id };
    };
    my $set_raw_child = sub {
        my ( $self, $child, $i ) = @_;
        $i = $self->last_index + 1 if not defined $i or $i == -1;
        $self->insert_at_index( $child, $i );    # XXX here we modify children
    };

    sub new_from_bioperl {
        my ( $class, $bpnode ) = @_;
        my $node = $class->new;

        # copy name
        my $name = $bpnode->id;
        $node->set_name($name) if defined $name;

        # copy branch length
        my $branch_length = $bpnode->branch_length;
        $node->set_branch_length($branch_length) if defined $branch_length;

        # copy description
        my $desc = $bpnode->description;
        $node->set_desc($desc) if defined $desc;

        # copy bootstrap
        my $bootstrap = $bpnode->bootstrap;
        $node->set_score($bootstrap)
          if defined $bootstrap and looks_like_number $bootstrap;

        # copy other tags
        for my $tag ( $bpnode->get_all_tags ) {
            my @values = $bpnode->get_tag_values($tag);
            $node->set_generic( $tag => \@values );
        }
        return $node;
    }

    sub prune_child {
        my ( $self, $child ) = @_;
        $self->delete($child);
        return $self;
    }

    sub collapse {
        my $self = shift;

        # can't collapse root
        if ( my $parent = $self->get_parent ) {

            # can't collapse terminal nodes
            if ( my @children = @{ $self->get_children } ) {

                # add node's branch length to that of children
                my $length = $self->get_branch_length;
                for my $child (@children) {
                    if ( defined $length ) {
                        my $child_length = $child->get_branch_length || 0;
                        $child->set_branch_length( $length + $child_length );
                    }

                    # attach children to node's parent
                    $child->set_parent($parent);
                }

                # prune node from parent
                $parent->prune_child($self);

                # delete node from tree
                if ( my $tree = $self->_get_container ) {
                    $tree->delete($self);
                }
            }
            else {
                return $self;
            }
        }
        else {
            return $self;
        }
    }

    sub set_parent {
        my ( $self, $parent ) = @_;
        if ( $parent and looks_like_object $parent, $TYPE_CONSTANT ) {
            $parent->set_child($self);
        }
        elsif ( not $parent ) {
            $set_raw_parent->($self);
        }
        return $self;
    }

    sub set_first_daughter {
        my ( $self, $fd ) = @_;
        $self->set_child( $fd, 0 );
        return $self;
    }

    sub set_last_daughter {
        my ( $self, $ld ) = @_;
        $self->set_child( $ld, scalar @{ $self->get_children } );
        return $self;
    }

    sub set_previous_sister {
        my ( $self, $ps ) = @_;
        if ( $ps and looks_like_object $ps, $TYPE_CONSTANT ) {
            if ( my $parent = $self->get_parent ) {
                my $children = $parent->get_children;
                my $j        = 0;
              FINDSELF: for ( my $i = $#{$children} ; $i >= 0 ; $i-- ) {
                    if ( $children->[$i] == $self ) {
                        $j = $i - 1;
                        last FINDSELF;
                    }
                }
                $j = 0 if $j == -1;
                $parent->set_child( $ps, $j );
            }
        }
        return $self;
    }

    sub set_next_sister {
        my ( $self, $ns ) = @_;
        if ( $ns and looks_like_object $ns, $TYPE_CONSTANT ) {
            if ( my $parent = $self->get_parent ) {
                my $children = $parent->get_children;
                my $last     = scalar @{$children};
                my $j        = $last;
              FINDSELF: for my $i ( 0 .. $#{$children} ) {
                    if ( $children->[$i] == $self ) {
                        $j = $i + 1;
                        last FINDSELF;
                    }
                }
                $parent->set_child( $ns, $j );
            }
        }
        return $self;
    }

    sub set_child {
        my ( $self, $child, $i ) = @_;

        # bad args?
        if ( not $child or not looks_like_object $child, $TYPE_CONSTANT ) {
            return;
        }

        # maybe nothing to do?
        if (   not $child
            or $child->get_id == $self->get_id
            or $child->is_child_of($self) )
        {
            return $self;
        }

        # $child_parent is NEVER $self, see above
        my $child_parent = $child->get_parent;

        # child is ancestor: this is obviously problematic, because
        # now we're trying to set a node nearer to the root on the
        # same lineage as the CHILD of a descendant. Because they're
        # on the same lineage it's hard to see how this can be done
        # sensibly. The decision here is to do:
        # 	1. we prune what is to become the parent (now the descendant)
        #	   from its current parent
        #	2. we set this pruned node (and its descendants) as a sibling
        #	   of what is to become the child
        #	3. we prune what is to become the child from its parent
        #	4. we set that pruned child as the child of $self
        if ( $child->is_ancestor_of($self) ) {

            # step 1.
            my $parent_parent = $self->get_parent;
            $parent_parent->prune_child($self);

            # step 2.
            $set_raw_parent->( $self, $child_parent );    # XXX could be undef
            if ($child_parent) {
                $set_raw_child->( $child_parent, $self );
            }
        }

        # step 3.
        if ($child_parent) {
            $child_parent->prune_child($child);
        }
        $set_raw_parent->( $child, $self );

        # now do the insert, first make room by shifting later siblings right
        my $children = $self->get_children;
        if ( defined $i ) {
            for ( my $j = $#{$children} ; $j >= 0 ; $j-- ) {
                my $sibling = $children->[$j];
                $set_raw_child->( $self, $sibling, $j + 1 );
            }
        }

        # no index was supplied, child becomes last daughter
        else {
            $i = scalar @{$children};
        }

        # step 4.
        $set_raw_child->( $self, $child, $i );
        return $self;
    }

    sub set_branch_length {
        my ( $self, $bl ) = @_;
        my $id = $self->get_id;
        if ( defined $bl && looks_like_number $bl && !ref $bl ) {
            $branch_length{$id} = $bl;
        }
        elsif ( defined $bl && ( !looks_like_number $bl || ref $bl ) ) {
            throw 'BadNumber' => "Branch length \"$bl\" is a bad number";
        }
        elsif ( !defined $bl ) {
            $branch_length{$id} = undef;
        }
        return $self;
    }

    sub set_node_below {
        my $self = shift;

        # can't set node below root
        if ( $self->is_root ) {
            return;
        }

        # instantiate new node from $self's class
        my $new_node = ( ref $self )->new(@_);

        # attach new node to $child's parent
        my $parent = $self->get_parent;
        $parent->set_child($new_node);

        # insert new node in tree
        # 		if ( my $tree = $self->_get_container ) {
        # 			$tree->insert( $new_node );
        # 		}
        # attach $self to new node
        $new_node->set_child($self);

        # done
        return $new_node;
    }

    # Example tree to illustrate rerooting algorithm:
    #
    #  A     B     C     D     E     F
    #   \   /     /     /     /     /
    #    \_/     /     /     /     /
    #     1     /     /     /     /
    # new->\   /     /     /     /
    #       \_/     /     /     /
    #        2     /     /     /
    #         \   /     /     /
    #          \_/     /     /
    #           3     /     /
    #            \   /     /
    #             \_/     /
    #              4     /
    #               \   /
    #                \_/
    #                 5
    #                 |
    sub set_root_below {
        my $self             = shift;
        my %constructor_args = @_;
        $constructor_args{'-name'} = 'root' if not $constructor_args{'-name'};

        # $self is node 5, nothing to do,
        # can't place root below root
        if ( $self->is_root ) {
            return;
        }
        my @ancestors = @{ $self->get_ancestors };

        # if @ancestors = ( 5 ); i.e. $self is node 4
        # root is already below $self
        if ( scalar @ancestors == 1 ) {
            return;
        }

        # let's say $self is node 1, ancestors is:
        # ( 2, 3, 4, 5 ) -> ( 2, 3, 4 )
        my $root = pop @ancestors;

        # ( 2, 3, 4 ) -> ( 2, 3 )
        my $node_above_root = pop @ancestors;

        # collapse node 4
        $node_above_root->collapse;

        # ( 2, 3 ) -> ( 2, 3, 5 ); 4 doesn't exist anymore (collapsed)
        push @ancestors, $root;

        # ( 2, 3, 5 ) -> ( new, 2, 3, 5 )
        unshift @ancestors, $self->set_node_below(%constructor_args);

        # i.e. $self wasn't 3
        if ( scalar @ancestors > 2 ) {
            for ( my $i = $#ancestors ; $i >= 0 ; $i-- ) {

                # flip parent & child
                $ancestors[$i]->set_child( $ancestors[ $i + 1 ] );
            }
        }
        else {

            # XXX
            $logger->info("Incomplete implementation reached");
            $ancestors[0]->set_child( $ancestors[1] );
        }
        if ( my $tree = $self->get_tree ) {
            $tree->insert( $ancestors[0] );
        }
        return $ancestors[0];
    }

    # 	sub set_root_below {
    # 		my $node = shift;
    # 		if ( $node->get_ancestors ) {
    # 			my @ancestors = @{ $node->get_ancestors };
    #
    # 			# first collapse root
    # 			my $root = $ancestors[-1];
    # 			my $lineage_containing_node;
    # 			my @children = @{ $root->get_children };
    # 		  FIND_LINEAGE: for my $child (@children) {
    # 				if ( $child->get_id == $node->get_id ) {
    # 					$lineage_containing_node = $child;
    # 					last FIND_LINEAGE;
    # 				}
    # 				for my $descendant ( @{ $child->get_descendants } ) {
    # 					if ( $descendant->get_id == $node->get_id ) {
    # 						$lineage_containing_node = $child;
    # 						last FIND_LINEAGE;
    # 					}
    # 				}
    # 			}
    # 			for my $child (@children) {
    # 				next if $child->get_id == $lineage_containing_node->get_id;
    # 				$child->set_parent($lineage_containing_node);
    # 			}
    #
    # 			# now create new root as parent of $node
    # 			my $newroot = __PACKAGE__->new( '-name' => 'root' );
    # 			$node->set_parent($newroot);
    #
    # 			# update list of ancestors, want to get rid of old root
    # 			# at $ancestors[-1] and have new root as $ancestors[0]
    # 			unshift @ancestors, $newroot;
    # 			pop @ancestors;
    #
    # 			# update connections
    # 			for ( my $i = $#ancestors ; $i >= 1 ; $i-- ) {
    # 				$ancestors[$i]->set_parent( $ancestors[ $i - 1 ] );
    # 			}
    #
    # 			# delete root if part of tree, insert new
    # 			if ( my $tree = $node->_get_container ) {
    # 				$tree->delete($root);
    # 				$tree->insert($newroot);
    # 			}
    # 		}
    # 	}

    sub set_tree {
        my ( $self, $tree ) = @_;
        my $id = $self->get_id;
        if ($tree) {
            if ( looks_like_object $tree, $CONTAINER_CONSTANT ) {
                $tree{$id} = $tree;
                weaken $tree{$id};
            }
            else {
                throw 'ObjectMismatch' => "$tree is not a tree";
            }
        }
        else {
            $tree{$id} = undef;
        }
        return $self;
    }

    sub get_parent { return $get_parent->(shift) }

    sub get_first_daughter {
        return $_[0]->get_child(0);
    }

    sub get_last_daughter {
        return $_[0]->get_child(-1);
    }

    sub get_previous_sister {
        my ($self) = @_;
        my $ps;
        if ( my $parent = $self->get_parent ) {
            my $children = $parent->get_children;
          FINDSELF: for ( my $i = $#{$children} ; $i >= 1 ; $i-- ) {
                if ( $children->[$i] == $self ) {
                    $ps = $children->[ $i - 1 ];
                    last FINDSELF;
                }
            }
        }
        return $ps;
    }

    sub get_next_sister {
        my ($self) = @_;
        my $ns;
        if ( my $parent = $self->get_parent ) {
            my $children = $parent->get_children;
          FINDSELF: for my $i ( 0 .. $#{$children} ) {
                if ( $children->[$i] == $self ) {
                    $ns = $children->[ $i + 1 ];
                    last FINDSELF;
                }
            }
        }
        return $ns;
    }

    sub get_branch_length { return $get_branch_length->(shift) }

    sub get_ancestors {
        my $self = shift;
        my @ancestors;
        my $node = $self;
        if ( $node = $node->get_parent ) {
            while ($node) {
                push @ancestors, $node;
                $node = $node->get_parent;
            }
            return \@ancestors;
        }
        else {
            return;
        }
    }

    sub get_root {
        my $self = shift;
        if ( my $anc = $self->get_ancestors ) {
            return $anc->[-1];
        }
        else {
            return $self;
        }
    }

    sub get_farthest_node {
        my ( $self, $patristic ) = @_;
        my $criterion = $patristic ? 'patristic' : 'nodal';
        my $method = sprintf 'calc_%s_distance', $criterion;
        my $root = $self->get_root;
        if ( my $terminals = $root->get_terminals ) {
            my ( $furthest_distance, $furthest_node ) = (0);
            for my $tip ( @{$terminals} ) {
                my $distance = $self->$method($tip);
                if ( $distance > $furthest_distance ) {
                    $furthest_distance = $distance;
                    $furthest_node     = $tip;
                }
            }
            return $furthest_node;
        }
    }

    sub get_sisters {
        my $self    = shift;
        my $sisters = $self->get_parent->get_children;
        return $sisters;
    }

    sub get_children { return $get_children->(shift) }

    sub get_child {
        my ( $self, $i ) = @_;
        $i = 0 if not defined $i;
        my $children = $self->get_children;
        return $children->[$i];
    }

    sub get_descendants {
        my $self    = shift;
        my @current = ($self);
        my @desc;
        while ( $self->_desc(@current) ) {
            @current = $self->_desc(@current);
            push @desc, @current;
        }
        return \@desc;
    }

    sub _desc {
        my $self    = shift;
        my @current = @_;
        my @return;
        foreach (@current) {
            my $children = $_->get_children;
            if ($children) {
                push @return, @{$children};
            }
        }
        return @return;
    }

    sub get_terminals {
        my $self = shift;
        if ( $self->is_terminal ) {
            return [$self];
        }
        else {
            my @terminals;
            my $desc = $self->get_descendants;
            if ( @{$desc} ) {
                foreach ( @{$desc} ) {
                    if ( $_->is_terminal ) {
                        push @terminals, $_;
                    }
                }
            }
            return \@terminals;
        }
    }

    sub get_internals {
        my $self = shift;
        my @internals;
        my $desc = $self->get_descendants;
        if ( @{$desc} ) {
            foreach ( @{$desc} ) {
                if ( $_->is_internal ) {
                    push @internals, $_;
                }
            }
        }
        return \@internals;
    }

    sub get_mrca {
        my ( $self, $other_node ) = @_;
        my $self_anc  = $self->get_ancestors       || [$self];
        my $other_anc = $other_node->get_ancestors || [$other_node];
        for my $i ( 0 .. $#{$self_anc} ) {
            my $self_anc_id = $self_anc->[$i]->get_id;
            for my $j ( 0 .. $#{$other_anc} ) {
                if ( $self_anc_id == $other_anc->[$j]->get_id ) {
                    return $self_anc->[$i];
                }
            }
        }
        $logger->warn( "using " . $self_anc->[-1]->get_internal_name );
        return $self_anc->[-1];
    }

    sub get_leftmost_terminal {
        my $self     = shift;
        my $daughter = $self;
      FIRST_DAUGHTER: while ($daughter) {
            if ( my $grand_daughter = $daughter->get_first_daughter ) {
                $daughter = $grand_daughter;
                next FIRST_DAUGHTER;
            }
            else {
                last FIRST_DAUGHTER;
            }
        }
        return $daughter;
    }

    sub get_rightmost_terminal {
        my $self     = shift;
        my $daughter = $self;
      LAST_DAUGHTER: while ($daughter) {
            if ( my $grand_daughter = $daughter->get_last_daughter ) {
                $daughter = $grand_daughter;
                next LAST_DAUGHTER;
            }
            else {
                last LAST_DAUGHTER;
            }
        }
        return $daughter;
    }

    sub get_tree {
        my $self = shift;
        my $id   = $self->get_id;
        return $tree{$id};
    }

    sub get_subtree {
        my $self = shift;
        my $tree = $fac->create_tree;
        $self->visit_depth_first(
            '-pre' => sub {
                my $node  = shift;
                my $clone = $node->clone;
                $node->set_generic( 'clone' => $clone );
                $tree->insert($clone);
                if ( my $parent = $node->get_parent ) {
                    if ( my $pclone = $node->get_generic('clone') ) {
                        $clone->set_parent($pclone);
                    }
                    else {
                        $clone->set_parent;
                    }
                }
            },
            '-post' => sub {
                my $node = shift;
                my $gen  = $node->get_generic;
                delete $gen->{'clone'};
            }
        );
        return $tree->_analyze;
    }

    sub is_terminal {
        return !shift->get_first_daughter;
    }

    sub is_internal {
        return !!shift->get_first_daughter;
    }

    sub is_preterminal {
        my $self     = shift;
        my $children = $self->get_children;
        for my $child ( @{$children} ) {
            return 0 if $child->is_internal;
        }
        return !!scalar @{$children};
    }

    sub is_first {
        return !shift->get_previous_sister;
    }

    sub is_last {
        return !shift->get_next_sister;
    }

    sub is_root {
        return !shift->get_parent;
    }

    sub is_descendant_of {
        my ( $self, $ancestor ) = @_;
        my $ancestor_id = $ancestor->get_id;
        while ($self) {
            if ( my $parent = $self->get_parent ) {
                $self = $parent;
            }
            else {
                return;
            }
            if ( $self->get_id == $ancestor_id ) {
                return 1;
            }
        }
    }

    sub is_ancestor_of {
        my ( $self, $child ) = @_;
        if ( $child->is_descendant_of($self) ) {
            return 1;
        }
        else {
            return;
        }
    }

    sub is_sister_of {
        my ( $self, $sister ) = @_;
        my ( $self_parent, $sister_parent ) =
          ( $self->get_parent, $sister->get_parent );
        if (   $self_parent
            && $sister_parent
            && $self_parent->get_id == $sister_parent->get_id )
        {
            return 1;
        }
        else {
            return;
        }
    }

    sub is_child_of {
        my ( $self, $node ) = @_;
        if ( my $parent = $self->get_parent ) {
            return $parent->get_id == $node->get_id;
        }
        return 0;
    }

    sub is_outgroup_of {
        my ( $outgroup, $nodes ) = @_;
        for my $i ( 0 .. $#{$nodes} ) {
            for my $j ( ( $i + 1 ) .. $#{$nodes} ) {
                my $mrca = $nodes->[$i]->get_mrca( $nodes->[$j] );
                return if $mrca->is_ancestor_of($outgroup);
            }
        }
        return 1;
    }

    sub can_contain {
        my $self = shift;
        my $type = $self->_type;
        for (@_) {
            return 0 if $type != $_->_type;
        }
        return 1;
    }

    sub calc_path_to_root {
        my $self = shift;
        my $node = $self;
        my $path = 0;
        while ($node) {
            my $branch_length = $node->get_branch_length;
            if ( defined $branch_length ) {
                $path += $branch_length;
            }
            if ( my $parent = $node->get_parent ) {
                $node = $parent;
            }
            else {
                last;
            }
        }
        return $path;
    }

    sub calc_nodes_to_root {
        my $self = shift;
        my ( $nodes, $parent ) = ( 0, $self );
        while ($parent) {
            $nodes++;
            $parent = $parent->get_parent;
            if ($parent) {
                if ( my $cntr = $parent->calc_nodes_to_root ) {
                    $nodes += $cntr;
                    last;
                }
            }
        }
        return $nodes;
    }

    sub calc_max_nodes_to_tips {
        my $self    = shift;
        my $self_id = $self->get_id;
        my ( $nodes, $maxnodes ) = ( 0, 0 );
        foreach my $child ( @{ $self->get_terminals } ) {
            $nodes = 0;
            while ( $child && $child->get_id != $self_id ) {
                $nodes++;
                $child = $child->get_parent;
            }
            if ( $nodes > $maxnodes ) {
                $maxnodes = $nodes;
            }
        }
        return $maxnodes;
    }

    sub calc_min_nodes_to_tips {
        my $self    = shift;
        my $self_id = $self->get_id;
        my ( $nodes, $minnodes );
        foreach my $child ( @{ $self->get_terminals } ) {
            $nodes = 0;
            while ( $child && $child->get_id != $self_id ) {
                $nodes++;
                $child = $child->get_parent;
            }
            if ( !$minnodes || $nodes < $minnodes ) {
                $minnodes = $nodes;
            }
        }
        return $minnodes;
    }

    sub calc_max_path_to_tips {
        my $self = shift;
        my $id   = $self->get_id;
        my ( $length, $maxlength ) = ( 0, 0 );
        foreach my $child ( @{ $self->get_terminals } ) {
            $length = 0;
            while ( $child && $child->get_id != $id ) {
                my $branch_length = $child->get_branch_length;
                if ( defined $branch_length ) {
                    $length += $branch_length;
                }
                $child = $child->get_parent;
            }
            if ( $length > $maxlength ) {
                $maxlength = $length;
            }
        }
        return $maxlength;
    }

    sub calc_min_path_to_tips {
        my $self = shift;
        my $id   = $self->get_id;
        my ( $length, $minlength );
        foreach my $child ( @{ $self->get_terminals } ) {
            $length = 0;
            while ( $child && $child->get_id != $id ) {
                my $branch_length = $child->get_branch_length;
                if ( defined $branch_length ) {
                    $length += $branch_length;
                }
                $child = $child->get_parent;
            }
            if ( !$minlength ) {
                $minlength = $length;
            }
            if ( $length < $minlength ) {
                $minlength = $length;
            }
        }
        return $minlength;
    }

    sub calc_patristic_distance {
        my ( $self, $other_node ) = @_;
        my $patristic_distance;
        my $mrca    = $self->get_mrca($other_node);
        my $mrca_id = $mrca->get_id;
        while ( $self->get_id != $mrca_id ) {
            my $branch_length = $self->get_branch_length;
            if ( defined $branch_length ) {
                $patristic_distance += $branch_length;
            }
            $self = $self->get_parent;
        }
        while ( $other_node and $other_node->get_id != $mrca_id ) {
            my $branch_length = $other_node->get_branch_length;
            if ( defined $branch_length ) {
                $patristic_distance += $branch_length;
            }
            $other_node = $other_node->get_parent;
        }
        return $patristic_distance;
    }

    sub calc_nodal_distance {
        my ( $self, $other_node ) = @_;
        my $nodal_distance;
        my $mrca    = $self->get_mrca($other_node);
        my $mrca_id = $mrca->get_id;
        while ( $self and $self->get_id != $mrca_id ) {
            $nodal_distance++;
            $self = $self->get_parent;
        }
        while ( $other_node and $other_node->get_id != $mrca_id ) {
            $nodal_distance++;
            $other_node = $other_node->get_parent;
        }
        return $nodal_distance;
    }

 #$tree->visit_depth_first(
 #	'-pre'            => sub { print "pre: ",            shift->get_name, "\n" },
 #	'-pre_daughter'   => sub { print "pre_daughter: ",   shift->get_name, "\n" },
 #	'-post_daughter'  => sub { print "post_daughter: ",  shift->get_name, "\n" },
 #	'-in'             => sub { print "in: ",             shift->get_name, "\n" },
 #	'-pre_sister'     => sub { print "pre_sister: ",     shift->get_name, "\n" },
 #	'-post_sister'    => sub { print "post_sister: ",    shift->get_name, "\n" },
 #	'-post'           => sub { print "post: ",           shift->get_name, "\n" },
 #	'-order'          => 'ltr',
 #);
    sub visit_depth_first {
        my $self = shift;
        my %args = looks_like_hash @_;

# 		my @keys = qw(pre pre_daughter post_daughter in pre_sister post_sister post order with_relatives);
# 		my %permitted_keys = map { "-${_}" => 1 } @keys;
# 		for my $key ( keys %args ) {
# 			if ( not exists $permitted_keys{$key} ) {
# 				throw 'BadArgs' => "Can't use argument $key";
# 			}
# 			if ( $key ne "-with_relatives" or $key ne "-order" ) {
# 				if ( not looks_like_instance $args{$key}, 'CODE' ) {
# 					throw 'BadArgs' => "Argument $key must be a code reference";
# 				}
# 			}
# 		}
        if ( $args{'-order'} and $args{'-order'} =~ /^rtl$/i ) {
            $args{'-sister_method'}   = 'get_previous_sister';
            $args{'-daughter_method'} = 'get_last_daughter';
        }
        else {
            $args{'-sister_method'}   = 'get_next_sister';
            $args{'-daughter_method'} = 'get_first_daughter';
        }
        $self->_visit_depth_first(%args);
        return $self;
    }

    sub _visit_depth_first {
        my ( $node, %args ) = @_;
        my ( $daughter_method, $sister_method ) =
          @args{qw(-daughter_method -sister_method)};
        $args{'-pre'}->($node) if $args{'-pre'};
        if ( my $daughter = $node->$daughter_method ) {
            my @args = ($node);
            push @args, $daughter if $args{'-with_relatives'};
            $args{'-pre_daughter'}->(@args) if $args{'-pre_daughter'};
            $daughter->_visit_depth_first(%args);
            $args{'-post_daughter'}->(@args) if $args{'-post_daughter'};
        }
        else {
            $args{'-no_daughter'}->($node) if $args{'-no_daughter'};
        }
        $args{'-in'}->($node) if $args{'-in'};
        if ( my $sister = $node->$sister_method ) {
            my @args = ($node);
            push @args, $sister if $args{'-with_relatives'};
            $args{'-pre_sister'}->(@args) if $args{'-pre_sister'};
            $sister->_visit_depth_first(%args);
            $args{'-post_sister'}->(@args) if $args{'-post_sister'};
        }
        else {
            $args{'-no_sister'}->($node) if $args{'-no_sister'};
        }
        $args{'-post'}->($node) if $args{'-post'};
    }

    sub visit_breadth_first {
        my $self = shift;
        my %args = looks_like_hash @_;
        if ( $args{'-order'} and $args{'-order'} =~ /rtl/i ) {
            $args{'-sister_method'}   = 'get_previous_sister';
            $args{'-daughter_method'} = 'get_last_daughter';
        }
        else {
            $args{'-sister_method'}   = 'get_next_sister';
            $args{'-daughter_method'} = 'get_first_daughter';
        }
        $self->_visit_breadth_first(%args);
        return $self;
    }

    sub _visit_breadth_first {
        my ( $node, %args ) = @_;
        my ( $daughter_method, $sister_method ) =
          @args{qw(-daughter_method -sister_method)};
        $args{'-pre'}->($node) if $args{'-pre'};
        if ( my $sister = $node->$sister_method ) {
            $args{'-pre_sister'}->($node) if $args{'-pre_sister'};
            $sister->_visit_breadth_first(%args);
            $args{'-post_sister'}->($node) if $args{'-post_sister'};
        }
        else {
            $args{'-no_sister'}->($node) if $args{'-no_sister'};
        }
        $args{'-in'}->($node) if $args{'-in'};
        if ( my $daughter = $node->$daughter_method ) {
            $args{'-pre_daughter'}->($node) if $args{'-pre_daughter'};
            $daughter->_visit_breadth_first(%args);
            $args{'-post_daughter'}->($node) if $args{'-post_daughter'};
        }
        else {
            $args{'-no_daughter'}->($node) if $args{'-no_daughter'};
        }
        $args{'-post'}->($node) if $args{'-post'};
    }

    sub visit_level_order {
        my ( $self, $sub ) = @_;
        if ( looks_like_instance $sub, 'CODE' ) {
            my @queue = ($self);
            while (@queue) {
                my $node = shift @queue;
                $sub->($node);
                if ( my $children = $node->get_children ) {
                    push @queue, @{$children};
                }
            }
        }
        else {
            throw 'BadArgs' => "'$sub' not a CODE reference";
        }
        return $self;
    }

    sub clone {
        my $self = shift;
        $logger->info("cloning $self");
        my %subs = @_;

        # we'll clone relatives in the tree, so no raw copying
        $subs{'set_parent'}          = sub { };
        $subs{'set_first_daughter'}  = sub { };
        $subs{'set_last_daughter'}   = sub { };
        $subs{'set_next_sister'}     = sub { };
        $subs{'set_previous_sister'} = sub { };
        $subs{'set_child'}           = sub { };
        $subs{'insert'}              = sub { };
        return $self->SUPER::clone(%subs);
    }

    sub to_xml {
        my $self  = shift;
        my @nodes = ( $self, @{ $self->get_descendants } );
        my $xml   = '';

        # first write out the node elements
        for my $node (@nodes) {
            if ( my $taxon = $node->get_taxon ) {
                $node->set_attributes( 'otu' => $taxon->get_xml_id );
            }
            if ( $node->is_root ) {
                $node->set_attributes( 'root' => 'true' );
            }
            $xml .= "\n" . $node->get_xml_tag(1);
        }

        # then the rootedge?
        if ( my $length = shift(@nodes)->get_branch_length ) {
            my $edge = $fac->create_xmlwritable(
                '-tag'        => 'rootedge',
                '-attributes' => {
                    'target' => $self->get_xml_id,
                    'id'     => "edge" . $self->get_id,
                    'length' => $length
                }
            );
            $xml .= "\n" . $edge->get_xml_tag(1);
        }

        # then the subtended edges
        for my $node (@nodes) {
            my $length = $node->get_branch_length;
            my $edge   = $fac->create_xmlwritable(
                '-tag'        => 'edge',
                '-attributes' => {
                    'source' => $node->get_parent->get_xml_id,
                    'target' => $node->get_xml_id,
                    'id'     => "edge" . $node->get_id
                }
            );
            $edge->set_attributes( 'length' => $length ) if defined $length;
            $xml .= "\n" . $edge->get_xml_tag(1);
        }
        return $xml;
    }

    {
        my ( $root_id, $string );

        #no warnings 'uninitialized';
        sub to_newick {
            my $node = shift;
            my %args = @_;
            $root_id = $node->get_id if not $root_id;
            my $blformat = '%f';

            # first create the name
            my $name;
            if ( $node->is_terminal or $args{'-nodelabels'} ) {
                if ( not $args{'-tipnames'} ) {
                    $name = $node->get_nexus_name;
                }
                elsif ( $args{'-tipnames'} =~ /^internal$/i ) {
                    $name = $node->get_nexus_name;
                }
                elsif ( $args{'-tipnames'} =~ /^taxon/i and $node->get_taxon ) {
                    if ( $args{'-tipnames'} =~ /^taxon_internal$/i ) {
                        $name = $node->get_taxon->get_nexus_name;
                    }
                    elsif ( $args{'-tipnames'} =~ /^taxon$/i ) {
                        $name = $node->get_taxon->get_nexus_name;
                    }
                }
                else {
                    $name = $node->get_generic( $args{'-tipnames'} );
                }
                if ( $args{'-translate'}
                    and exists $args{'-translate'}->{$name} )
                {
                    $name = $args{'-translate'}->{$name};
                }
            }

            # now format branch length
            my $branch_length;
            if ( defined( $branch_length = $node->get_branch_length ) ) {
                if ( $args{'-blformat'} ) {
                    $blformat = $args{'-blformat'};
                }
                $branch_length = sprintf $blformat, $branch_length;
            }

            # now format nhx
            my $nhx;
            if ( $args{'-nhxkeys'} ) {
                my $sep;
                if ( $args{'-nhxstyle'} =~ /^mesquite$/i ) {
                    $sep = ',';
                    $nhx = '[%';
                }
                else {
                    $sep = ':';
                    $nhx = '[&&NHX:';
                }
                my @nhx;
                for my $i ( 0 .. $#{ $args{'-nhxkeys'} } ) {
                    my $key   = $args{'-nhxkeys'}->[$i];
                    my $value = $node->get_generic($key);
                    push @nhx, " $key = $value " if $value;
                }
                if (@nhx) {
                    $nhx .= join $sep, @nhx;
                    $nhx .= ']';
                }
                else {
                    $nhx = '';
                }
            }

            # recurse further
            if ( my $first_daughter = $node->get_first_daughter ) {
                $string .= '(';
                $first_daughter->to_newick(%args);
            }

            # append to growing newick string
            $string .= ')'                  if $node->get_first_daughter;
            $string .= $name                if defined $name;
            $string .= ':' . $branch_length if defined $branch_length;
            $string .= $nhx                 if $nhx;
            if ( $root_id == $node->get_id ) {
                undef $root_id;
                my $result = $string . ';';
                undef $string;
                return $result;
            }

            # recurse further
            elsif ( my $next_sister = $node->get_next_sister ) {
                $string .= ',';
                $next_sister->to_newick(%args);
            }
        }
    }

    sub to_dom {
        my ( $self, $dom ) = shift;
        $dom ||= $Bio::Phylo::NeXML::DOM::DOM;
        unless ( looks_like_object $dom, _DOMCREATOR_ ) {
            throw 'BadArgs' => 'DOM factory object not provided';
        }
        my @nodes = ( $self, @{ $self->get_descendants } );
        my @elts;

        # first write out the node elements
        for my $node (@nodes) {
            if ( my $taxon = $node->get_taxon ) {
                $node->set_attributes( 'otu' => $taxon->get_xml_id );
            }
            if ( $node->is_root ) {
                $node->set_attributes( 'root' => 'true' );
            }
            push @elts, $node->get_dom_elt($dom);
        }

        # then the rootedge?
        if ( my $length = shift(@nodes)->get_branch_length ) {
            my $target = $self->get_xml_id;
            my $id     = "edge" . $self->get_id;
            my $elt    = $dom->create_element(
                '-tag'        => 'rootedge',
                '-attributes' => {
                    'target' => $target,
                    'id'     => $id,
                    'length' => $length,
                }
            );
            push @elts, $elt;
        }

        # then the subtended edges
        for my $node (@nodes) {
            my $source = $node->get_parent->get_xml_id;
            my $target = $node->get_xml_id;
            my $id     = "edge" . $node->get_id;
            my $length = $node->get_branch_length;
            my $elt    = $dom->create_element(
                '-tag'        => 'edge',
                '-attributes' => {
                    'source' => $source,
                    'target' => $target,
                    'id'     => $id,
                }
            );
            $elt->set_attributes( 'length' => $length ) if ( defined $length );
            push @elts, $elt;
        }
        return @elts;
    }

    sub _cleanup {
        my $self = shift;
        my $id   = $self->get_id;
        for my $field (@fields) {
            delete $field->{$id};
        }
    }

    sub _type { $TYPE_CONSTANT }
    sub _tag  { 'node' }

    sub _container { $CONTAINER_CONSTANT }

    # podinherit_insert_token

}
1;
__DATA__

sub add_Descendent{
   my ( $self,$child ) = @_;
   $self->set_child( $child );
   return scalar @{ $self->get_children };
}

sub each_Descendent{
	my $self = shift;
	if ( my $children = $self->get_children ) {
		return @{ $children };
   	}
   	return;
}

sub get_all_Descendents{
	my $self = shift;
	if ( my $desc = $self->get_descendants ) {
		return @{ $desc };
	}
	return;
}

*get_Descendents = \&get_all_Descendents;

*is_Leaf = \&is_terminal;
*is_otu = \&is_terminal;

sub descendent_count{
	my $self = shift;
	my $count = 0;
	if ( my $desc = get_descendants ) {
		$count = scalar @{ $desc };
	}
	return $count;
}

sub height{ shift->calc_max_path_to_tips }

sub depth{ shift->calc_path_to_root }

sub branch_length{
	my $self = shift;
	if ( @_ ) {
		$self->set_branch_length(shift);
	}
	return $self->get_branch_length;
}

sub id {
    my $self = shift;
    if ( @_ ) {
    	$self->set_name(shift);
    }
    return $self->get_name;
}

sub internal_id { shift->get_id }

sub description {
	my $self = shift;
	if ( @_ ) {
		$self->set_desc(shift);
	}
	return $self->get_desc;
}

sub bootstrap {
	my ( $self, $bs ) = @_;
	if ( defined $bs && looks_like_number $bs ) {
		$self->set_score($bs);
	}
	return $self->get_score;
}

sub ancestor {
	my $self = shift;
	if ( @_ ) {
		$self->set_parent(shift);
	}
	return $self->get_parent;
}

sub invalidate_height { }

sub add_tag_value{
	my $self = shift;
	if ( @_ ) {
		my ( $key, $value ) = @_;
		$self->set_generic( $key, $value );
	}
	return 1;
}

sub remove_tag {
	my ( $self, $tag ) = @_;
	my %hash = %{ $self->get_generic };
	my $exists = exists $hash{$tag};
	delete $hash{$tag};
	$self->set_generic();
	$self->set_generic(%hash);
	return !!$exists;
}

sub remove_all_tags{ shift->set_generic() }

sub get_all_tags {
	my $self = shift;
	my %hash = %{ $self->get_generic };
	return keys %hash;
}

sub get_tag_values{
	my ( $self, $tag ) = @_;
	my $values = $self->get_generic($tag);
	return ref $values ? @{ $values } : $values;
}

sub has_tag{
	my ( $self, $tag ) = @_;
	my %hash = %{ $self->get_generic };
	return exists $hash{$tag};
}

sub id_output { shift->get_internal_name }