| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::Assembly::IO - Handler for Assembly::IO Formats
use Bio::Assembly::IO;
$in = Bio::Assembly::IO->new(-file=>"<inputfilename",
-format=>'phrap');
$out = Bio::Assembly::IO->new(-file=>">outputfilename",
-format=>'phrap');
while ( my $scaffold = $in->next_assembly() ) {
# do something with Bio::Assembly::Scaffold instance
# ...
$out->write_assembly(-scaffold => $scaffold);
}
$in->close;
$out->close;
Bio::Assembly::IO is a handler module for formats in the Assembly::IO set (e.g. Bio::Assembly::IO::phrap).
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://redmine.open-bio.org/projects/bioperl/
Robson Francisco de Souza
E-mail: rfsouza@citri.iq.usp.br
#
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new
Usage : $stream = Bio::Assembly::IO->new( -file => $filename,
-format =>'format' )
Function: Returns a new assembly stream
Returns : A Bio::Assembly::IO::Handler initialised
with the appropriate format
Args : -file => $filename
-format => format
Title : next_assembly Usage : $scaffold = $stream->next_assembly() Function: Reads the next assembly object from the stream and returns it. Returns : a Bio::Assembly::ScaffoldI compliant object Args : none
Title : next_contig Usage : $contig = $stream->next_contig() Function: Reads the next contig or singlet from the stream and returns it. Returns : a Bio::Assembly::Contig or Bio::Contig::Assembly::Singlet Args : none
Title : write_assembly
Usage : $stream->write_assembly($assembly)
Function: Write the assembly object in desired format. This method calls
write_header(), write_contigs() and write_footer() internally.
Returns : 1 on success, 0 for error
Args : A Bio::Assembly::Scaffold object
Title : write_header
Usage : $stream->write_header($assembly)
Function: Write the start of the assembly file. It can be called at any time,
not when starting to write the assembly file.
Returns : 1 on success, 0 for error
Args : A Bio::Assembly::Scaffold object or ... (check the specific format
driver for more details)
Title : write_contig Usage : $stream->write_contig($contig) Function: Write a contig object in the desired format. Returns : 1 on success, 0 for error Args : A Bio::Assembly::Contig object
Title : _load_format_module Usage : *INTERNAL Assembly::IO stuff* Function: Loads up (like use) a module at run time on demand Example : Returns : Args :
Title : _guess_format
Usage : $obj->_guess_format($filename)
Function: guess format based on file suffix
Example :
Returns : guessed format of filename (lower case)
Args :
Notes : formats that _filehandle() will guess includes
ace, phrap and tigr at the moment
Title : _sort
Usage : @sorted_values = $ass_io->_sort(@values)
Function: Sort a list of values naturally if Sort::Naturally is installed
(nicer), lexically otherwise (not as nice, but safe)
Returns : array of sorted values
Args : array of values to sort
| BioPerl documentation | Contained in the BioPerl distribution. |
# $Id: IO.pm 16690 2010-01-14 07:27:29Z kortsch $ # # BioPerl module for Bio::Assembly::IO # # based on the Bio::SeqIO module # by Ewan Birney <birney@ebi.ac.uk> # and Lincoln Stein <lstein@cshl.org> # # Copyright Robson Francisco de Souza # # You may distribute this module under the same terms as perl itself # # _history # POD documentation - main docs before the code
package Bio::Assembly::IO; use strict; use base qw(Bio::Root::Root Bio::Root::IO);
sub new { my ($caller,@args) = @_; my $class = ref($caller) || $caller; # or do we want to call SUPER on an object if $caller is an # object? if( $class =~ /Bio::Assembly::IO::(\S+)/ ) { my ($self) = $class->SUPER::new(@args); $self->_initialize(@args); return $self; } else { my %param = @args; @param{ map { lc $_ } keys %param } = values %param; # lowercase keys $class->throw("Need at least a file name to proceed!") unless (defined $param{'-file'} || defined $ARGV[0]); my $format = $param{'-format'} || $class->_guess_format( $param{-file} || $ARGV[0] ); $format = "\L$format"; # normalize capitalization to lower case if ($format =~ /-/) { ($format, my $variant) = split('-', $format, 2); push @args, (-variant => $variant); } return unless( $class->_load_format_module($format) ); return "Bio::Assembly::IO::$format"->new(@args); } } # _initialize is chained for all SeqIO classes sub _initialize { my($self, @args) = @_; # initialize the IO part $self->_initialize_io(@args); }
sub next_assembly { my ($self) = @_; $self->throw("Cannot read from a generic Bio::Assembly::IO object."); }
sub next_contig { my ($self) = @_; $self->throw("Cannot read from a generic Bio::Assembly::IO object."); }
sub write_assembly { my ($self, @args) = @_; my ($scaf, $write_singlets) = $self->_rearrange([qw(SCAFFOLD SINGLETS)], @args); # Sanity check if ( !$scaf || !$scaf->isa('Bio::Assembly::ScaffoldI') ) { $self->throw("Must provide a Bio::Assembly::Scaffold object when calling write_assembly"); } # Write header $self->write_header($scaf); # ID-sorted contig and read entries my @contig_ids = $scaf->get_contig_ids; if ($write_singlets) { push @contig_ids, $scaf->get_singlet_ids; } @contig_ids = _sort(@contig_ids); # Write contigs for my $contig_id ( @contig_ids ) { my $contig = $scaf->get_contig_by_id($contig_id) || $scaf->get_singlet_by_id($contig_id); $self->write_contig($contig); } # Write footer $self->write_footer($scaf); return 1; }
sub write_header { my ($self) = @_; $self->throw("Cannot write from a generic Bio::Assembly::IO object."); }
sub write_contig { my ($self) = @_; $self->throw("Cannot write from a generic Bio::Assembly::IO object."); }
sub write_footer { my ($self) = @_; $self->throw("Cannot write from a generic Bio::Assembly::IO object."); }
sub _load_format_module { my ($self,$format) = @_; my $module = "Bio::Assembly::IO::" . $format; my $ok; eval { $ok = $self->_load_module($module); }; if ( $@ ) { print STDERR <<END; $self: could not load $format - for more details on supported formats please see the Assembly::IO docs Exception $@ END ; } return $ok; }
sub _guess_format { my $class = shift; my $arg = shift; return unless defined($arg); return 'ace' if ($arg =~ /\.ace/i); return 'phrap' if ($arg =~ /\.phrap/i); return 'tigr' if ($arg =~ /\.tigr/i); return 'maq' if ($arg =~ /\.maq/i); return 'sam' if ($arg =~ /\.[bs]am/i); return 'bowtie' if ($arg =~ /\.bowtie/i); }
sub _sort { my @arr = @_; my @sorted_arr; if (eval { require Sort::Naturally }) { @sorted_arr = Sort::Naturally::nsort( @arr ); # natural sort (better) } else { @sorted_arr = sort @arr; # lexical sort (safe) } return @sorted_arr; } sub DESTROY { my $self = shift; $self->close(); } # I need some direction on these!! The module works so I haven't fiddled with them! # Me neither! (rfsouza) sub TIEHANDLE { my ($class,$val) = @_; return bless {'seqio' => $val}, $class; } sub READLINE { my $self = shift; return $self->{'seqio'}->next_seq() unless wantarray; my (@list, $obj); push @list, $obj while $obj = $self->{'seqio'}->next_seq(); return @list; } sub PRINT { my $self = shift; $self->{'seqio'}->write_seq(@_); } 1;