| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::DB::Flat::BDB - Interface for BioHackathon standard BDB-indexed flat file
#You should not be using this module directly.
See Bio::DB::Flat.
This object provides the basic mechanism to associate positions in files with primary and secondary name spaces. Unlike Bio::Index::Abstract (see Bio::Index::Abstract), this is specialized to work with the BerkeleyDB-indexed "common" flat file format worked out at the 2002 BioHackathon.
This object is the guts to the mechanism, which will be used by the specific objects inheriting from it.
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web:
https://redmine.open-bio.org/projects/bioperl/
Email - lstein@cshl.org
The rest of the documentation details each of the object methods. Internal methods are usually preceded with an "_" (underscore).
Title : get_PrimarySeq_stream
Usage : $stream = get_PrimarySeq_stream
Function: Makes a Bio::DB::SeqStreamI compliant object
which provides a single method, next_primary_seq
Returns : Bio::DB::SeqStreamI
Args : none
Title : get_all_primary_ids
Usage : @ids = $seqdb->get_all_primary_ids()
Function: gives an array of all the primary_ids of the
sequence objects in the database.
Example :
Returns : an array of strings
Args : none
The following methods MUST be implemented by subclasses.
The following methods MAY be overridden by subclasses.
| BioPerl documentation | Contained in the BioPerl distribution. |
# # # BioPerl module for Bio::DB::Flat::BDB # # Please direct questions and support issues to <bioperl-l@bioperl.org> # # Cared for by Lincoln Stein <lstein@cshl.org> # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code
# Let the code begin... package Bio::DB::Flat::BDB; use strict; use DB_File; use IO::File; use Fcntl qw(O_CREAT O_RDWR O_RDONLY); use File::Spec; use Bio::SeqIO; use Bio::DB::RandomAccessI; use Bio::Root::Root; use Bio::Root::IO; use base qw(Bio::DB::Flat); sub _initialize { my $self = shift; my ($max_open) = $self->_rearrange(['MAXOPEN'],@_); $self->{bdb_maxopen} = $max_open || 32; } # return a filehandle seeked to the appropriate place # this only works with the primary namespace sub _get_stream { my ($self,$id) = @_; my ($filepath,$offset,$length) = $self->_lookup_primary($id) or $self->throw("Unable to find a record for $id in the flat file index"); my $fh = $self->_fhcache($filepath) or $self->throw("couldn't open $filepath: $!"); seek($fh,$offset,0) or $self->throw("can't seek on $filepath: $!"); $fh; } # return records corresponding to the indicated index # if there are multiple hits will return a list in list context, # otherwise will throw an exception sub fetch_raw { my ($self,$id,$namespace) = @_; # secondary lookup if (defined $namespace && $namespace ne $self->primary_namespace) { my @hits = $self->_lookup_secondary($namespace,$id); $self->throw("Multiple records correspond to $namespace=>$id but function called in a scalar context") unless wantarray; return map {$self->_read_record(@$_)} @hits; } # primary lookup my @args = $self->_lookup_primary($id) or $self->throw("Unable to find a record for $id in the flat file index"); return $self->_read_record(@args); } # create real live Bio::Seq object sub get_Seq_by_id { my $self = shift; my $id = shift; my $fh = eval {$self->_get_stream($id)} or return; my $seqio = $self->{bdb_cached_parsers}{fileno $fh} ||= Bio::SeqIO->new( -Format => $self->file_format, -fh => $fh); return $seqio->next_seq; } # fetch array of Bio::Seq objects sub get_Seq_by_acc { my $self = shift; unshift @_,'ACC' if @_==1; my ($ns,$key) = @_; my @primary_ids = $self->expand_ids($ns => $key); $self->throw("more than one sequences correspond to this accession") if @primary_ids > 1 && ! wantarray; my @rc = map {$self->get_Seq_by_id($_)} @primary_ids; return wantarray ? @rc : $rc[0]; } # fetch array of Bio::Seq objects sub get_Seq_by_version { my $self = shift; unshift @_,'VERSION' if @_==1; my ($ns,$key) = @_; my @primary_ids = $self->expand_ids($ns => $key); $self->throw("more than one sequences correspond to this accession") if @primary_ids > 1 && !wantarray; my @rc = map {$self->get_Seq_by_id($_)} @primary_ids; return wantarray ? @rc : $rc[0]; }
sub get_PrimarySeq_stream { my $self = shift; my @files = $self->files || 0; my $out = Bio::SeqIO::MultiFile->new( -format => $self->file_format , -files => \@files); return $out; } sub get_all_primary_ids { my $self = shift; my $db = $self->primary_db; return keys %$db; }
# this will perform an ID lookup on a (possibly secondary) # id, returning all the corresponding ids sub expand_ids { my $self = shift; my ($ns,$key) = @_; return $key unless defined $ns; return $key if $ns eq $self->primary_namespace; my $db = $self->secondary_db($ns) or $self->throw("invalid secondary namespace $ns"); my $record = $db->{$key} or return; # nothing there return $self->unpack_secondary($record); } # build index from files listed sub build_index { my $self = shift; my @files = @_; my $count = 0; for my $file (@files) { $file = File::Spec->rel2abs($file) unless File::Spec->file_name_is_absolute($file); $count += $self->_index_file($file); } $self->write_config; $count; } sub _index_file { my $self = shift; my $file = shift; my $fileno = $self->_path2fileno($file); defined $fileno or $self->throw("could not create a file number for $file"); my $fh = $self->_fhcache($file) or $self->throw("could not open $file for indexing: $!"); my $offset = 0; my $count = 0; while (!eof($fh)) { my ($ids,$adjustment) = $self->parse_one_record($fh) or next; $adjustment ||= 0; # prevent uninit variable warning my $pos = tell($fh) + $adjustment; $self->_store_index($ids,$file,$offset,$pos-$offset); $offset = $pos; $count++; } $count; }
sub default_primary_namespace { return "ACC"; } sub default_secondary_namespaces { return; } sub _read_record { my $self = shift; my ($filepath,$offset,$length) = @_; my $fh = $self->_fhcache($filepath) or $self->throw("couldn't open $filepath: $!"); seek($fh,$offset,0) or $self->throw("can't seek on $filepath: $!"); my $record; read($fh,$record,$length) or $self->throw("can't read $filepath: $!"); $record } # return a list in the form ($filepath,$offset,$length) sub _lookup_primary { my $self = shift; my $primary = shift; my $db = $self->primary_db or $self->throw("no primary namespace database is open"); my $record = $db->{$primary} or return; # nothing here my($fileid,$offset,$length) = $self->unpack_primary($record); my $filepath = $self->_fileno2path($fileid) or $self->throw("no file path entry for fileid $fileid"); return ($filepath,$offset,$length); } # return a list of array refs in the form [$filepath,$offset,$length] sub _lookup_secondary { my $self = shift; my ($namespace,$secondary) = @_; my @primary = $self->expand_ids($namespace=>$secondary); return map {[$self->_lookup_primary($_)]} @primary; } # store indexing information into a primary & secondary record # $namespaces is one of: # 1. a scalar corresponding to the primary name # 2. a hashref corresponding to namespace=>id identifiers # it is valid for secondary id to be an arrayref sub _store_index { my $self = shift; my ($keys,$filepath,$offset,$length) = @_; my ($primary,%secondary); if (ref $keys eq 'HASH') { my %valid_secondary = map {$_=>1} $self->secondary_namespaces; while (my($ns,$value) = each %$keys) { if ($ns eq $self->primary_namespace) { $primary = $value; } else { $valid_secondary{$ns} or $self->throw("invalid secondary namespace $ns"); push @{$secondary{$ns}},$value; } } $primary or $self->throw("no primary namespace ID provided"); } else { $primary = $keys; } $self->throw("invalid primary ID; must be a scalar") if ref($primary) =~ /^(ARRAY|HASH)$/; # but allow stringified objects $self->_store_primary($primary,$filepath,$offset,$length); for my $ns (keys %secondary) { my @ids = ref $secondary{$ns} ? @{$secondary{$ns}} : $secondary{$ns}; $self->_store_secondary($ns,$_,$primary) foreach @ids; } 1; } # store primary index sub _store_primary { my $self = shift; my ($id,$filepath,$offset,$length) = @_; my $db = $self->primary_db or $self->throw("no primary namespace database is open"); my $fileno = $self->_path2fileno($filepath); defined $fileno or $self->throw("could not create a file number for $filepath"); my $record = $self->pack_primary($fileno,$offset,$length); $db->{$id} = $record or return; # nothing here 1; } # store a primary index name under a secondary index sub _store_secondary { my $self = shift; my ($secondary_ns,$secondary_id,$primary_id) = @_; my $db = $self->secondary_db($secondary_ns) or $self->throw("invalid secondary namespace $secondary_ns"); # first get whatever secondary ids are already stored there my @primary = $self->unpack_secondary($db->{$secondary_id}); # uniqueify my %unique = map {$_=>undef} @primary,$primary_id; my $record = $self->pack_secondary(keys %unique); $db->{$secondary_id} = $record; } # get output file handle sub _outfh { my $self = shift; #### XXXXX FINISH ##### # my $ } # unpack a primary record into fileid,offset,length sub unpack_primary { my $self = shift; my $index_record = shift; return split "\t",$index_record; } # unpack a secondary record into a list of primary ids sub unpack_secondary { my $self = shift; my $index_record = shift or return; return split "\t",$index_record; } # pack a list of fileid,offset,length into a primary id record sub pack_primary { my $self = shift; my ($fileid,$offset,$length) = @_; return join "\t",($fileid,$offset,$length); } # pack a list of primary ids into a secondary id record sub pack_secondary { my $self = shift; my @secondaries = @_; return join "\t",@secondaries; } sub primary_db { my $self = shift; # lazy opening $self->_open_bdb unless exists $self->{bdb_primary_db}; return $self->{bdb_primary_db}; } sub secondary_db { my $self = shift; my $secondary_namespace = shift or $self->throw("usage: secondary_db(\$secondary_namespace)"); $self->_open_bdb unless exists $self->{bdb_primary_db}; return $self->{bdb_secondary_db}{$secondary_namespace}; } sub _open_bdb { my $self = shift; my $flags = $self->write_flag ? O_CREAT|O_RDWR : O_RDONLY; my $primary_db = {}; tie(%$primary_db,'DB_File',$self->_catfile($self->_primary_db_name),$flags,0666,$DB_BTREE) or $self->throw("Could not open primary index file: $! (did you remember to use -write_flag=>1?)"); $self->{bdb_primary_db} = $primary_db; for my $secondary ($self->secondary_namespaces) { my $secondary_db = {}; tie(%$secondary_db,'DB_File',$self->_catfile($self->_secondary_db_name($secondary)),$flags,0666,$DB_BTREE) or $self->throw("Could not open primary index file"); $self->{bdb_secondary_db}{$secondary} = $secondary_db; } 1; } sub _primary_db_name { my $self = shift; my $pns = $self->primary_namespace or $self->throw('no primary namespace defined'); return "key_$pns"; } sub _secondary_db_name { my $self = shift; my $sns = shift; return "id_$sns"; } sub _fhcache { my $self = shift; my $path = shift; my $write = shift; if (!$self->{bdb_fhcache}{$path}) { $self->{bdb_curopen} ||= 0; if ($self->{bdb_curopen} >= $self->{bdb_maxopen}) { my @lru = sort {$self->{bdb_cacheseq}{$a} <=> $self->{bdb_cacheseq}{$b};} keys %{$self->{bdb_fhcache}}; splice(@lru, $self->{bdb_maxopen} / 3); $self->{bdb_curopen} -= @lru; for (@lru) { delete $self->{bdb_fhcache}{$_} } } if ($write) { my $modifier = $self->{bdb_fhcache_seenit}{$path}++ ? '>' : '>>'; $self->{bdb_fhcache}{$path} = IO::File->new("${modifier}${path}") or return; } else { $self->{bdb_fhcache}{$path} = IO::File->new($path) or return; } $self->{bdb_curopen}++; } $self->{bdb_cacheseq}{$path}++; $self->{bdb_fhcache}{$path} } 1;