| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::DB::SeqFeature::Store::Loader -- Loader
# non-instantiable base class
This is the base class for Bio::DB::SeqFeature::Loader::GFF3Loader, Bio::DB::SeqFeature::Loader::GFFLoader, and Bio::DB::SeqFeature::FeatureFileLoader. Please see the manual pages for these modules.
Title : new Usage : $loader = Bio::DB::SeqFeature::Store::GFF3Loader->new(@options) Function: create a new parser Returns : a Bio::DB::SeqFeature::Store::GFF3Loader gff3 parser and loader Args : several - see below Status : public
This method creates a new GFF3 loader and establishes its connection with a Bio::DB::SeqFeature::Store database. Arguments are -name=>$value pairs as described in this table:
Name Value
---- -----
-store A writable Bio::DB::SeqFeature::Store database handle.
-seqfeature_class The name of the type of Bio::SeqFeatureI object to create
and store in the database (Bio::DB::SeqFeature by default)
-sf_class A shorter alias for -seqfeature_class
-verbose Send progress information to standard error.
-fast If true, activate fast loading (see below)
-chunk_size Set the storage chunk size for nucleotide/protein sequences
(default 2000 bytes)
-tmp Indicate a temporary directory to use when loading non-normalized
features.
-map_coords A code ref that will transform a list of ($ref,[$start1,$end1]...)
coordinates into a list of ($newref,[$newstart1,$newend1]...)
-index_subfeatures Indicate true if subfeatures should be indexed. Default is true.
-summary_stats Rebuild summary stats at the end of loading (not incremental,
so takes a long time)
When you call new(), a connection to a Bio::DB::SeqFeature::Store database should already have been established and the database initialized (if appropriate).
Some combinations of Bio::SeqFeatures and Bio::DB::SeqFeature::Store databases support a fast loading mode. Currently the only reliable implementation of fast loading is the combination of DBI::mysql with Bio::DB::SeqFeature. The other important restriction on fast loading is the requirement that a feature that contains subfeatures must occur in the GFF3 file before any of its subfeatures. Otherwise the subfeatures that occurred before the parent feature will not be attached to the parent correctly. This restriction does not apply to normal (slow) loading.
If you use an unnormalized feature class, such as Bio::SeqFeature::Generic, then the loader needs to create a temporary database in which to cache features until all their parts and subparts have been seen. This temporary databases uses the "berkeleydb" adaptor. The -tmp option specifies the directory in which that database will be created. If not present, it defaults to the system default tmp directory specified by File::Spec->tmpdir().
The -chunk_size option allows you to tune the representation of DNA/Protein sequence in the Store database. By default, sequences are split into 2000 base/residue chunks and then reassembled as needed. This avoids the problem of pulling a whole chromosome into memory in order to fetch a short subsequence from somewhere in the middle. Depending on your usage patterns, you may wish to tune this parameter using a chunk size that is larger or smaller than the default.
Title : load Usage : $count = $loader->load(@ARGV) Function: load the indicated files or filehandles Returns : number of feature lines loaded Args : list of files or filehandles Status : public
Once the loader is created, invoke its load() method with a list of GFF3 or FASTA file paths or previously-opened filehandles in order to load them into the database. Compressed files ending with .gz, .Z and .bz2 are automatically recognized and uncompressed on the fly. Paths beginning with http: or ftp: are treated as URLs and opened using the LWP GET program (which must be on your path).
FASTA files are recognized by their initial ">" character. Do not feed the loader a file that is neither GFF3 nor FASTA; I don't know what will happen, but it will probably not be what you expect.
The following read-only accessors return values passed or created during new():
store() the long-term Bio::DB::SeqFeature::Store object
tmp_store() the temporary Bio::DB::SeqFeature::Store object used
during loading
sfclass() the Bio::SeqFeatureI class
fast() whether fast loading is active
seq_chunk_size() the sequence chunk size
verbose() verbose progress messages
The following methods are used internally and may be overidden by subclasses.
$class = $loader->default_seqfeature_class
Return the default SeqFeatureI class (Bio::DB::SeqFeature).
$flag = $loader->subfeatures_normalized([$new_flag])
Get or set a flag that indicates that the subfeatures are normalized. This is deduced from the SeqFeature class information.
$flag = $loader->subfeatures_in_table([$new_flag])
Get or set a flag that indicates that feature/subfeature relationships are stored in a table. This is deduced from the SeqFeature class and Store information.
$count = $loader->load_fh($filehandle)
Load the GFF3 data at the other end of the filehandle and return true if successful. Internally, load_fh() invokes:
start_load(); do_load($filehandle); finish_load();
These methods are called at the start and end of a filehandle load.
$loader->build_summary
Call this to rebuild the summary coverage statistics. This is done automatically if new() was passed a true value for -summary_stats at create time.
$count = $loader->do_load($fh)
This is called by load_fh() to load the GFF3 file's filehandle and return the number of lines loaded.
$loader->load_line($data);
Load a line of a GFF3 file. You must bracket this with calls to start_load() and finish_load()!
$loader->start_load();
$loader->load_line($_) while <FH>;
$loader->finish_load();
$loader->handle_feature($data_line)
This method is called to process a single data line. It manipulates information stored a data structure called $self->{load_data}.
$loader->handle_meta($data_line)
This method is called to process a single data line. It manipulates information stored a data structure called $self->{load_data}.
$loader->store_current_feature()
This method is called to store the currently active feature in the database. It uses a data structure stored in $self->{load_data}.
($reserved,$unreserved) = $loader->parse_attributes($attribute_line)
This method parses the information contained in the $attribute_line into two hashrefs, one containing the values of reserved attribute tags (e.g. ID) and the other containing the values of unreserved ones.
$loader->start_or_finish_sequence('Chr9')
This method is called at the beginning and end of a fasta section.
$loader->load_sequence('gatttcccaaa')
This method is called to load some amount of sequence after start_or_finish_sequence() is first called.
my $io_file = $loader->open_fh($filehandle_or_path)
This method opens up the indicated file or pipe, using some intelligence to recognized compressed files and URLs and doing the right thing.
my $ids = $loader->loaded_ids; my $id_cnt = @$ids;
After performing a load, this returns an array ref containing all the feature primary ids that were created during the load.
my $ids = $self->local_ids; my $id_cnt = @$ids;
After performing a load, this returns an array ref containing all the load file IDs that were contained within the file just loaded.
my $time = $loader->time
This method returns the current time in seconds, using Time::HiRes if available.
my $unescaped = GFF3Loader::unescape($escaped)
This is an internal utility. It is the same as CGI::Util::unescape, but doesn't change pluses into spaces and ignores unicode escapes.
This is an early version, so there are certainly some bugs. Please use the BioPerl bug tracking system to report bugs.
bioperl, Bio::DB::SeqFeature::Store, Bio::DB::SeqFeature::Segment, Bio::DB::SeqFeature::NormalizedFeature, Bio::DB::SeqFeature::Store::GFF3Loader, Bio::DB::SeqFeature::Store::DBI::mysql, Bio::DB::SeqFeature::Store::berkeleydb
Lincoln Stein <lstein@cshl.org>.
Copyright (c) 2006 Cold Spring Harbor Laboratory.
This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself.
| BioPerl documentation | Contained in the BioPerl distribution. |
package Bio::DB::SeqFeature::Store::Loader;
# load utility - incrementally load the store based on GFF3 file # # two modes: # slow mode -- features can occur in any order in the GFF3 file # fast mode -- all features with same ID must be contiguous in GFF3 file use strict; use Carp 'croak'; use IO::File; use Bio::DB::GFF::Util::Rearrange; use Bio::DB::SeqFeature::Store; use File::Spec; use File::Temp 'tempdir'; use base 'Bio::Root::Root'; use constant DEFAULT_SEQ_CHUNK_SIZE => 2000;
sub new { my $self = shift; my ($store,$seqfeature_class,$tmpdir,$verbose,$fast, $seq_chunk_size,$coordinate_mapper,$index_subfeatures,$summary_stats) = rearrange(['STORE', ['SF_CLASS','SEQFEATURE_CLASS'], ['TMP','TMPDIR'], 'VERBOSE', 'FAST', 'CHUNK_SIZE', 'MAP_COORDS', 'INDEX_SUBFEATURES', 'SUMMARY_STATS' ],@_); $seqfeature_class ||= $self->default_seqfeature_class; eval "require $seqfeature_class" unless $seqfeature_class->can('new'); $self->throw($@) if $@; my $normalized = $seqfeature_class->can('subfeatures_are_normalized') && $seqfeature_class->subfeatures_are_normalized; my $in_table = $seqfeature_class->can('subfeatures_are_stored_in_a_table') && $seqfeature_class->subfeatures_are_stored_in_a_table; if ($fast) { my $canfast = $normalized && $in_table; warn <<END unless $canfast; Only features that support the Bio::DB::SeqFeature::NormalizedTableFeature interface can be loaded using the -fast method. Reverting to slower feature-by-feature method. END $fast &&= $canfast; } # try to bring in highres time() function eval "require Time::HiRes"; $tmpdir ||= File::Spec->tmpdir(); my ($tmp_store,$temp_load); unless ($normalized) { # remember the temporary directory in order to delete it on exit $temp_load = tempdir( 'BioDBSeqFeature_XXXXXXX', DIR=>$tmpdir, CLEANUP=>1 ); $tmp_store = Bio::DB::SeqFeature::Store->new(-adaptor => 'berkeleydb', -temporary=> 1, -dsn => $temp_load, -cache => 1, -write => 1) unless $normalized; } $index_subfeatures = 1 unless defined $index_subfeatures; return bless { store => $store, tmp_store => $tmp_store, seqfeature_class => $seqfeature_class, fast => $fast, seq_chunk_size => $seq_chunk_size || DEFAULT_SEQ_CHUNK_SIZE, verbose => $verbose, load_data => {}, tmpdir => $tmpdir, temp_load => $temp_load, subfeatures_normalized => $normalized, subfeatures_in_table => $in_table, coordinate_mapper => $coordinate_mapper, index_subfeatures => $index_subfeatures, summary_stats => $summary_stats, },ref($self) || $self; } sub coordinate_mapper { my $self = shift; my $d = $self->{coordinate_mapper}; $self->{coordinate_mapper} = shift if @_; $d; } sub index_subfeatures { my $self = shift; my $d = $self->{index_subfeatures}; $self->{index_subfeatures} = shift if @_; $d; } sub summary_stats { my $self = shift; my $d = $self->{summary_stats}; $self->{summary_stats} = shift if @_; $d; }
sub load { my $self = shift; my $start = $self->time(); my $count = 0; for my $file_or_fh (@_) { $self->msg("loading $file_or_fh...\n"); my $fh = $self->open_fh($file_or_fh) or $self->throw("Couldn't open $file_or_fh: $!"); $count += $self->load_fh($fh); $self->msg(sprintf "load time: %5.2fs\n",$self->time()-$start); } if ($self->summary_stats) { $self->msg("Building summary statistics for coverage graphs..."); my $start = $self->time(); $self->build_summary; $self->msg(sprintf "coverage graph build time: %5.2fs\n",$self->time()-$start); } $self->msg(sprintf "total load time: %5.2fs\n",$self->time()-$start); $count; }
sub store { shift->{store} } sub tmp_store { shift->{tmp_store} } sub sfclass { shift->{seqfeature_class} } sub fast { shift->{fast} } sub seq_chunk_size { shift->{seq_chunk_size} } sub verbose { shift->{verbose} }
sub default_seqfeature_class { my $self = shift; return 'Bio::DB::SeqFeature'; }
sub subfeatures_normalized { my $self = shift; my $d = $self->{subfeatures_normalized}; $self->{subfeatures_normalized} = shift if @_; $d; }
sub subfeatures_in_table { my $self = shift; my $d = $self->{subfeatures_in_table}; $self->{subfeatures_in_table} = shift if @_; $d; }
sub load_fh { my $self = shift; my $fh = shift; $self->start_load(); my $count = $self->do_load($fh); $self->finish_load(); $count; }
sub start_load { my $self = shift; $self->create_load_data; $self->store->start_bulk_update() if $self->fast; } sub create_load_data { my $self = shift; $self->{load_data}{CurrentFeature} = undef; $self->{load_data}{CurrentID} = undef; $self->{load_data}{IndexIt} = {}; $self->{load_data}{Local2GlobalID} = {}; $self->{load_data}{count} = 0; $self->{load_data}{mode} = undef; $self->{load_data}{start_time} = 0; } sub delete_load_data { my $self = shift; delete $self->{load_data}; } sub finish_load { my $self = shift; $self->store_current_feature(); # during fast loading, we will have a feature left at the very end $self->start_or_finish_sequence(); # finish any half-loaded sequences if ($self->fast) { $self->{load_data}{start_time} = $self->time(); $self->store->finish_bulk_update; } $self->msg(sprintf "%5.2fs\n",$self->time()-$self->{load_data}{start_time}); eval {$self->store->commit}; # don't delete load data so that caller can ask for the loaded IDs # $self->delete_load_data; }
sub build_summary { my $self = shift; $self->store->build_summary_statistics; }
sub do_load { my $self = shift; my $fh = shift; $self->{load_data}{start_time} = $self->time(); $self->{load_data}->{millenium_time} = $self->{load_data}{start_time}; $self->load_line($_) while <$fh>; $self->msg(sprintf "%d features loaded in %5.2fs%s\r", $self->{load_data}->{count}, $self->time()-$self->{load_data}{start_time}, ' 'x80 ); $self->{load_data}{count}; }
sub load_line { my $self = shift; my $line = shift; # don't do anything }
sub handle_feature { my $self = shift; my $line = shift; # do nothing }
sub handle_meta { my $self = shift; my $line = shift; # do nothing } sub _indexit { my $self = shift; my $id = shift; $id ||= ''; # avoid uninit warnings my $indexhash = $self->{load_data}{IndexIt}; $indexhash->{$id} = shift if @_; return $indexhash->{$id}; } sub _local2global { my $self = shift; my $id = shift; $id ||= ''; # avoid uninit warnings my $indexhash = $self->{load_data}{Local2GlobalID}; $indexhash->{$id} = shift if @_; return $indexhash->{$id}; }
sub store_current_feature { my $self = shift; my $ld = $self->{load_data}; defined $ld->{CurrentFeature} or return; my $f = $ld->{CurrentFeature}; my $normalized = $self->subfeatures_normalized; my $indexed = $self->_indexit($ld->{CurrentID}); # logic is as follows: # 1. If the feature is an indexed feature, then we store it into the main database # so that it can be searched. It doesn't matter whether it is a top-level feature # or a subfeature. # 2. If the feature class is normalized, but not indexed, then we store it into the # main database using the "no_index" method. This will make it accessible to # queries on the top level parent, but it won't come up by itself in range or # attribute searches. # 3. Otherwise, this is an unindexed subfeature; we store it in the temporary database # until the object build step, at which point it gets integrated into its object tree # and copied into the main database. if ($indexed) { $self->store->store($f); } elsif ($normalized) { $self->store->store_noindex($f) } else { $self->tmp_store->store_noindex($f) } my $id = $f->primary_id; # assigned by store() $self->_local2global($ld->{CurrentID} => $id); $self->_indexit($ld->{CurrentID} => 0)if $normalized; # no need to remember this undef $ld->{CurrentID}; undef $ld->{CurrentFeature}; }
sub parse_attributes { my $self = shift; my $att = shift; # do nothing }
# this gets called at the beginning and end of a fasta section sub start_or_finish_sequence { my $self = shift; my $seqid = shift; if (my $sl = $self->{fasta_load}) { if (defined $sl->{seqid}) { $self->store->insert_sequence($sl->{seqid},$sl->{sequence},$sl->{offset}); delete $self->{fasta_load}; } } if (defined $seqid) { $self->{fasta_load} = {seqid => $seqid, offset => 0, sequence => ''}; } }
sub load_sequence { my $self = shift; my $seq = shift; my $sl = $self->{fasta_load} or return; my $cs = $self->seq_chunk_size; $sl->{sequence} .= $seq; while (length $sl->{sequence} >= $cs) { my $chunk = substr($sl->{sequence},0,$cs); $self->store->insert_sequence($sl->{seqid},$chunk,$sl->{offset}); $sl->{offset} += length $chunk; substr($sl->{sequence},0,$cs) = ''; } }
sub open_fh { my $self = shift; my $thing = shift; no strict 'refs'; return $thing if defined fileno($thing); return IO::File->new("gunzip -c $thing |") if $thing =~ /\.gz$/; return IO::File->new("uncompress -c $thing |") if $thing =~ /\.Z$/; return IO::File->new("bunzip2 -c $thing |") if $thing =~ /\.bz2$/; return IO::File->new("GET $thing |") if $thing =~ /^(http|ftp):/; return $thing if ref $thing && $thing->isa('IO::String'); return IO::File->new($thing); } sub msg { my $self = shift; my @msg = @_; return unless $self->verbose; print STDERR @msg; }
sub loaded_ids { my $self = shift; my @ids = values %{$self->{load_data}{Local2GlobalID}} if $self->{load_data}; return \@ids; }
sub local_ids { my $self = shift; my @ids = keys %{$self->{load_data}{Local2GlobalID}} if $self->{load_data}; return \@ids; }
sub time { return Time::HiRes::time() if Time::HiRes->can('time'); return time(); }
sub unescape { my $self = shift; my $todecode = shift; $todecode =~ s/%([0-9a-fA-F]{2})/chr hex($1)/ge; return $todecode; } sub DESTROY { my $self = shift; if (my $ld = $self->{temp_load}) { unlink $ld; } } 1; __END__