Index

NAME
SYNOPSIS
DESCRIPTION
FEEDBACK
AUTHOR - Gaurav Gupta
CONTRIBUTORS
PROJECT LEADERS
PROJECT DESCRIPTION
APPENDIX
Access Methods

Code Index:

package Bio::Map::Physical
EOF

NAME

Bio::Map::Physical - A class for handling a Physical Map (such as FPC)

SYNOPSIS

    use Bio::MapIO;

    # accquire a Bio::Map::Physical using Bio::MapIO::fpc
    my $mapio = Bio::MapIO->new(-format => "fpc",-file => "rice.fpc",
                               -readcor => 0);

    my $physical = $mapio->next_map();

    # get all the markers ids
    foreach my $marker ( $physical->each_markerid() ) {
      print "Marker $marker\n";

      # acquire the marker object using Bio::Map::FPCMarker
      my $markerobj = $physical->get_markerobj($marker);

      # get all the clones hit by this marker
      foreach my $clone ($markerobj->each_cloneid() ) {
          print " +++$clone\n";
      }
  }

DESCRIPTION

This class is basically a continer class for a collection of Contig maps and other physical map information.

Bio::Map::Physical has been tailored to work for FPC physical maps, but could probably be used for others as well (with the appropriate MapIO module).

This class also has some methods with specific functionalities:

  print_gffstyle()     : Generates GFF; either Contigwise[Default] or
                         Groupwise

  print_contiglist()   : Prints the list of Contigs, markers that hit the
                         contig, the global position and whether the marker
                         is a placement (<P>) or a Framework (<F>) marker.

  print_markerlist()   : Prints the markers list; contig and corresponding
                         number of clones.

  matching_bands()     : Given two clones [and tolerence], this method
                         calculates how many matching bands do they have.

  coincidence_score()  : Given two clones [,tolerence and gellen], this
                         method calculates the Sulston Coincidence score.

For faster access and better optimization, the data is stored internally in hashes. The corresponding objects are created on request.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://redmine.open-bio.org/projects/bioperl/

AUTHOR - Gaurav Gupta

Email gaurav@genome.arizona.edu

CONTRIBUTORS

Sendu Bala bix@sendu.me.uk

PROJECT LEADERS

Jamie Hatfield jamie@genome.arizona.edu Dr. Cari Soderlund cari@genome.arizona.edu

PROJECT DESCRIPTION

The project was done in Arizona Genomics Computational Laboratory (AGCoL) at University of Arizona.

This work was funded by USDA-IFAFS grant #11180 titled "Web Resources for the Computation and Display of Physical Mapping Data".

For more information on this project, please refer: http://www.genome.arizona.edu

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

Access Methods

These methods let you get and set the member variables

version

 Title   : version
 Usage   : my $version = $map->version();
 Function: Get/set the version of the program used to
           generate this map
 Returns : scalar representing the version
 Args    : none to get, OR string to set

modification_user

 Title   : modification_user
 Usage   : my $modification_user = $map->modification_user();
 Function: Get/set the name of the user who last modified this map
 Returns : scalar representing the username
 Args    : none to get, OR string to set

group_type

 Title   : group_type
 Usage   : $map->group_type($grptype);
	       my $grptype = $map->group_type();
 Function: Get/set the group type of this map
 Returns : scalar representing the group type
 Args    : none to get, OR string to set

group_abbr

 Title   : group_abbr
 Usage   : $map->group_abbr($grpabbr);
	       my $grpabbr = $map->group_abbr();
 Function: get/set the group abbrev of this map
 Returns : string representing the group abbrev
 Args    : none to get, OR string to set

core_exists

 Title   : core_exists
 Usage   : my $core_exists = $map->core_exists();
 Function: Get/set if the FPC file is accompanied by COR file
 Returns : boolean
 Args    : none to get, OR 1|0 to set

each_cloneid

 Title   : each_cloneid
 Usage   : my @clones = $map->each_cloneid();
 Function: returns an array of clone names
 Returns : list of clone names
 Args    : none

get_cloneobj

 Title   : get_cloneobj
 Usage   : my $cloneobj = $map->get_cloneobj('CLONEA');
 Function: returns an object of the clone given in the argument
 Returns : object of the clone
 Args    : scalar representing the clone name

each_markerid

 Title   : each_markerid
 Usage   : my @markers = $map->each_markerid();
 Function: returns list of marker names
 Returns : list of marker names
 Args    : none

get_markerobj

 Title   : get_markerobj
 Usage   : my $markerobj = $map->get_markerobj('MARKERA');
 Function: returns an object of the marker given in the argument
 Returns : object of the marker
 Args    : scalar representing the marker name

each_contigid

 Title   : each_contigid
 Usage   : my @contigs = $map->each_contigid();
 Function: returns a list of contigs (numbers)
 Returns : list of contigs
 Args    : none

get_contigobj

 Title   : get_contigobj
 Usage   : my $contigobj = $map->get_contigobj('CONTIG1');
 Function: returns an object of the contig given in the argument
 Returns : object of the contig
 Args    : scalar representing the contig number

matching_bands

 Title   : matching_bands
 Usage   : $self->matching_bands('cloneA','cloneB',[$tol]);
 Function: given two clones [and tolerence], this method calculates how many
           matching bands do they have.
           (this method is ported directly from FPC)
 Returns : scalar representing the number of matching bands
 Args    : names of the clones ('cloneA', 'cloneB') [Default tolerence=7]

coincidence_score

 Title   : coincidence_score
 Usage   : $self->coincidence_score('cloneA','cloneB'[,$tol,$gellen]);
 Function: given two clones [,tolerence and gellen], this method calculates
           the Sulston Coincidence score.
           (this method is ported directly from FPC)
 Returns : scalar representing the Sulston coincidence score.
 Args    : names of the clones ('cloneA', 'cloneB')
           [Default tol=7 gellen=3300.0]

print_contiglist

 Title   : print_contiglist
 Usage   : $map->print_contiglist([showall]); #[Default 0]
 Function: prints the list of contigs, markers that hit the contig, the
           global position and whether the marker is a placement (P) or
           a Framework (F) marker.
 Returns : none
 Args    : [showall] [Default 0], 1 includes all the discrepant markers

print_markerlist

 Title    : print_markerlist
 Usage    : $map->print_markerlist();
 Function : prints the marker list; contig and corresponding number of
            clones for each marker.
 Returns  : none
 Args     : none

print_gffstyle

 Title    : print_gffstyle
 Usage    : $map->print_gffstyle([style]);
 Function : prints GFF; either Contigwise (default) or Groupwise
 Returns  : none
 Args     : [style] default = 0 contigwise, else
                              1 groupwise (chromosome-wise).

_calc_markerposition

 Title   : _calc_markerposition
 Usage   : $map->_calc_markerposition();
 Function: Calculates the position of the marker in the contig
 Returns : none
 Args    : none

_calc_contigposition

 Title   : _calc_contigposition
 Usage   : $map->_calc_contigposition();
 Function: calculates the position of the contig in the group
 Returns : none
 Args    : none

_calc_contiggroup

 Title   : _calc_contiggroup
 Usage   : $map->_calc_contiggroup();
 Function: calculates the group of the contig
 Returns : none
 Args    : none

_set<Type>Ref

 Title   : _set<Type>Ref
 Usage   : These are used for initializing the reference of the hash in
           Bio::MapIO (fpc.pm) to the corresponding hash in Bio::Map
           (physical.pm). Should be used only from Bio::MapIO System.
               $map->setCloneRef(\%_clones);
               $map->setMarkerRef(\%_markers);
               $map->setContigRef(\%_contigs);
 Function: sets the hash references to the corresponding hashes
 Returns : none
 Args    : reference of the hash.