| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::PopGen::IO::prettybase - Extract individual allele data from PrettyBase format
Do not use directly, use through the Bio::PopGen::IO driver
This object will parse comma delimited PrettyBase output. PrettyBase is defined by the SeattleSNPs http://pga.gs.washington.edu/
This is expected to be tab delimited (you can vary with the field_delimiter flag SITE SAMPLE ALLELE1 ALLELE2
There are 2 initialization parameters, the delimiter (-field_delimiter) [default 'tab'] and a boolean -no_header which specifies if there is no header line to read in. All lines starting with '#' will be skipped
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://redmine.open-bio.org/projects/bioperl/
Email jason-at-bioperl.org
Matthew Hahn, matthew.hahn-at-duke.edu
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new
Usage : my $obj = Bio::PopGen::IO::prettybase->new();
Function: Builds a new Bio::PopGen::IO::prettybase object
Returns : an instance of Bio::PopGen::IO::prettybase
Args : -field_delimiter => a field delimiter character or regexp (default is /\t/ )
-header => boolean if the file will have a header and parser should
skip first line in the file (default is false)
-convert_indel_states => convert alleles which are longer than one character
to an 'I' meaning insert state, and alleles which are
'-' to a delete state.
(default is false)
Title : flag
Usage : $obj->flag($flagname,$newval)
Function: Get/Set the flag value
Returns : value of a flag (a boolean)
Args : A flag name, currently we expect
'header', 'field_delimiter', or 'allele_delimiter'
on set, new value (a boolean or undef, optional)
Title : next_individual Usage : my $ind = $popgenio->next_individual; Function: Retrieve the next individual from a dataset Returns : Bio::PopGen::IndividualI object Args : none
Title : next_population Usage : my $ind = $popgenio->next_population; Function: Retrieve the next population from a dataset Returns : Bio::PopGen::PopulationI object Args : none Note : Many implementation will not implement this
Title : write_individual Usage : $popgenio->write_individual($ind); Function: Write an individual out in the file format Returns : none Args : L<Bio::PopGen::PopulationI> object(s)
Title : write_population Usage : $popgenio->write_population($pop); Function: Write a population out in the file format Returns : none Args : L<Bio::PopGen::PopulationI> object(s) Note : Many implementation will not implement this
| BioPerl documentation | Contained in the BioPerl distribution. |
# # BioPerl module for Bio::PopGen::IO::prettybase # # Please direct questions and support issues to <bioperl-l@bioperl.org> # # Cared for by Jason Stajich <jason-at-bioperl.org> # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code
# Let the code begin... package Bio::PopGen::IO::prettybase; use vars qw($FieldDelim $Header); use strict; ($FieldDelim,$Header) =( '\t',0); use Bio::PopGen::Individual; use Bio::PopGen::Population; use Bio::PopGen::Genotype; use base qw(Bio::PopGen::IO);
sub _initialize { my($self, @args) = @_; my ($fieldsep, $conv_indels, $header) = $self->_rearrange([qw(FIELD_DELIMITER CONVERT_INDEL_STATES HEADER)],@args); $self->flag('header', defined $header ? $header : $Header); $self->flag('field_delimiter',defined $fieldsep ? $fieldsep : $FieldDelim); $self->{'_header'} = undef; $self->{'_parsed_individiuals'} = []; $self->{'_parsed'} = 0; $self->flag('convert_indel',$conv_indels || 0); return 1; }
sub flag{ my $self = shift; my $fieldname = shift; return unless defined $fieldname; return $self->{'_flag'}->{$fieldname} = shift if @_; return $self->{'_flag'}->{$fieldname}; }
sub next_individual { my ($self) = @_; unless( $self->{'_parsed'} ) { $self->_parse_prettybase; } return $self->{'_parsed_individiuals'}->[$self->{'_iterator'}++]; }
# Plan is to just return the whole dataset as a single population by # default I think - people would then have each population in a separate # file. sub next_population{ my ($self) = @_; my @inds; while( my $ind = $self->next_individual ) { push @inds, $ind; } return unless @inds; Bio::PopGen::Population->new(-individuals => \@inds); } sub _parse_prettybase { my $self = shift; my %inds; my $convert_indels = $self->flag('convert_indel'); while( defined( $_ = $self->_readline) ) { next if( /^\s*\#/ || /^\s+$/ || ! length($_) ); my ($site,$sample,@alleles) = split($self->flag('field_delimiter'),$_); if( ! defined $sample ) { warn("sample id is undefined for $_"); next; } for my $allele ( @alleles ) { $allele =~ s/^\s+//; $allele =~ s/\s+$//; if( $convert_indels ) { if( length($allele) > 1 ) { # we have an insert state $allele = 'I'; } elsif( $allele eq '-' ) { # have a delete state $allele = 'D'; } } } my $g = Bio::PopGen::Genotype->new(-alleles => \@alleles, -marker_name => $site, -individual_id=> $sample); if( ! defined $inds{$sample} ) { $inds{$sample} = Bio::PopGen::Individual->new(-unique_id => $sample); } $inds{$sample}->add_Genotype($g); } $self->{'_parsed_individiuals'} = [ values %inds ]; $self->{'_parsed'} = 1; return; }
sub write_individual{ my ($self,@inds) = @_; foreach my $ind ( @inds ) { if (! ref($ind) || ! $ind->isa('Bio::PopGen::IndividualI') ) { $self->warn("Cannot write an object that is not a Bio::PopGen::IndividualI object"); next; } foreach my $marker ( $ind->get_marker_names ) { my $g = $ind->get_Genotypes(-marker=> $marker); next unless defined $g; $self->_print( join("\t", $marker, $ind->unique_id, $g->get_Alleles), "\n"); } } }
sub write_population{ my ($self,@pops) = @_; foreach my $pop ( @pops ) { if (! ref($pop) || ! $pop->isa('Bio::PopGen::PopulationI') ) { $self->warn("Cannot write an object that is not a Bio::PopGen::PopulationI object"); next; } my @mnames = $pop->get_marker_names; foreach my $ind ( $pop->get_Individuals ) { if (! ref($ind) || ! $ind->isa('Bio::PopGen::IndividualI') ) { $self->warn("Cannot write an object that is not a Bio::PopGen::IndividualI object"); next; } foreach my $marker ( @mnames ) { my $g = $ind->get_Genotypes(-marker=> $marker); next unless defined $g; $self->_print( join("\t", $marker, $ind->unique_id, $g->get_Alleles), "\n"); } } } } 1;