| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::Search::Hit::BlastPullHit - A parser and hit object for BLASTN hits
# generally we use Bio::SearchIO to build these objects
use Bio::SearchIO;
my $in = Bio::SearchIO->new(-format => 'blast_pull',
-file => 'result.blast');
while (my $result = $in->next_result) {
while (my $hit = $result->next_hit) {
print $hit->name, "\n";
print $hit->score, "\n";
print $hit->significance, "\n";
while (my $hsp = $hit->next_hsp) {
# process HSPI objects
}
}
}
This object implements a parser for BLASTN hit output.
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://redmine.open-bio.org/projects/bioperl/
Email bix@sendu.me.uk
Additional contributors names and emails here
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new
Usage : my $obj = Bio::Search::Hit::BlastNHit->new();
Function: Builds a new Bio::Search::Hit::BlastNHit object.
Returns : Bio::Search::Hit::BlastNHit
Args : -chunk => [Bio::Root::IO, $start, $end] (required if no -parent)
-parent => Bio::PullParserI object (required if no -chunk)
-hit_data => array ref with [name description score significance]
where the array ref provided to -chunk contains an IO object
for a filehandle to something representing the raw data of the
hit, and $start and $end define the tell() position within the
filehandle that the hit data starts and ends (optional; defaults
to start and end of the entire thing described by the filehandle)
Title : next_hsp
Usage : while( $hsp = $obj->next_hsp()) { ... }
Function : Returns the next available High Scoring Pair
Example :
Returns : L<Bio::Search::HSP::HSPI> object or null if finished
Args : none
Usage : $hit_object->hsps(); Purpose : Get a list containing all HSP objects. Example : @hsps = $hit_object->hsps(); Returns : list of L<Bio::Search::HSP::BlastHSP> objects. Argument : none
Usage : $hit_object->hsp( [string] );
Purpose : Get a single HSPI object for the present HitI object.
Example : $hspObj = $hit_object->hsp; # same as 'best'
: $hspObj = $hit_object->hsp('best');
: $hspObj = $hit_object->hsp('worst');
Returns : Object reference for a L<Bio::Search::HSP::HSPI> object.
Argument : String (or no argument).
: No argument (default) = highest scoring HSP (same as 'best').
: 'best' = highest scoring HSP.
: 'worst' = lowest scoring HSP.
Throws : Exception if an unrecognized argument is used.
See Also : hsps() (hsps), num_hsps()
Title : rewind
Usage : $result->rewind;
Function: Allow one to reset the HSP iterator to the beginning, so that
next_hsp() will subsequently return the first hsp and so on.
Returns : n/a
Args : none
| BioPerl documentation | Contained in the BioPerl distribution. |
# # BioPerl module for Bio::Search::Hit::BlastPullHit # # Please direct questions and support issues to <bioperl-l@bioperl.org> # # Cared for by Sendu Bala <bix@sendu.me.uk> # # Copyright Sendu Bala # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code
# Let the code begin... package Bio::Search::Hit::BlastPullHit; use strict; use Bio::Search::HSP::BlastPullHSP; use base qw(Bio::Root::Root Bio::Search::Hit::PullHitI);
sub new { my ($class, @args) = @_; my $self = $class->SUPER::new(@args); $self->_setup(@args); my $fields = $self->_fields; foreach my $field (qw( header start_end )) { $fields->{$field} = undef; } my $hit_data = $self->_raw_hit_data; if ($hit_data && ref($hit_data) eq 'ARRAY') { foreach my $field (qw(name description score significance)) { $fields->{$field} = shift(@{$hit_data}); } } $self->_dependencies( { ( name => 'header', length => 'header', description => 'header', accession => 'header', next_hsp => 'header', query_start => 'start_end', query_end => 'start_end', hit_start => 'start_end', hit_end => 'start_end' ) } ); return $self; } # # PullParserI discovery methods so we can answer all HitI questions # sub _discover_header { my $self = shift; $self->_chunk_seek(0); my $header = $self->_get_chunk_by_end("\n Score = "); unless ($header) { # no alignment or other data; all information was in the hit table of # the result $self->_calculate_accession_from_name; $self->_fields->{header} = 1; return; } $self->{_after_header} = $self->_chunk_tell; ($self->_fields->{name}, $self->_fields->{description}, $self->_fields->{length}) = $header =~ /^(\S+)\s+(\S.+?)?\s+Length\s*=\s*(\d+)/sm; if ($self->_fields->{description}) { $self->_fields->{description} =~ s/\n//g; } else { $self->_fields->{description} = ''; } $self->_calculate_accession_from_name; $self->_fields->{header} = 1; } sub _calculate_accession_from_name { my $self = shift; my $name = $self->get_field('name'); if ($name =~ /.+?\|.+?\|.+?\|(\w+)/) { $self->_fields->{accession} = $1; } elsif ($self->_fields->{name} =~ /.+?\|(\w+)?\./) { # old form? $self->_fields->{accession} = $1; } else { $self->_fields->{accession} = $name; } } sub _discover_start_end { my $self = shift; my ($q_start, $q_end, $h_start, $h_end); foreach my $hsp ($self->hsps) { my ($this_q_start, $this_h_start) = $hsp->start; my ($this_q_end, $this_h_end) = $hsp->end; if (! defined $q_start || $this_q_start < $q_start) { $q_start = $this_q_start; } if (! defined $h_start || $this_h_start < $h_start) { $h_start = $this_h_start; } if (! defined $q_end || $this_q_end > $q_end) { $q_end = $this_q_end; } if (! defined $h_end || $this_h_end > $h_end) { $h_end = $this_h_end; } } $self->_fields->{query_start} = $q_start; $self->_fields->{query_end} = $q_end; $self->_fields->{hit_start} = $h_start; $self->_fields->{hit_end} = $h_end; } sub _discover_next_hsp { my $self = shift; my $pos = $self->{_end_of_previous_hsp} || $self->{_after_header}; return unless $pos; $self->_chunk_seek($pos); my ($start, $end) = $self->_find_chunk_by_end("\n Score = "); if ((defined $end && ($end + $self->_chunk_true_start) > $self->_chunk_true_end) || ! $end) { $start = $self->{_end_of_previous_hsp} || $self->{_after_header}; $end = $self->_chunk_true_end; } else { $end += $self->_chunk_true_start; } $start += $self->_chunk_true_start; return if $start >= $self->_chunk_true_end; $self->{_end_of_previous_hsp} = $end - $self->_chunk_true_start; #*** needs to inherit piped_behaviour, and we need to deal with _sequential # ourselves $self->_fields->{next_hsp} = Bio::Search::HSP::BlastPullHSP->new(-parent => $self, -chunk => [$self->chunk, $start, $end]); } sub _discover_num_hsps { my $self = shift; $self->_fields->{num_hsps} = $self->hsps; }
sub next_hsp { my $self = shift; my $hsp = $self->get_field('next_hsp'); undef $self->_fields->{next_hsp}; return $hsp; }
sub hsps { my $self = shift; my $old = $self->{_end_of_previous_hsp}; $self->rewind; my @hsps; while (defined(my $hsp = $self->next_hsp)) { push(@hsps, $hsp); } $self->{_end_of_previous_hsp} = $old; return @hsps; }
sub hsp { my ($self, $type) = @_; $type ||= 'best'; $self->throw_not_implemented; }
sub rewind { my $self = shift; delete $self->{_end_of_previous_hsp}; } # have p() a synonym of significance() sub p { return shift->significance; } 1;