Bio::SearchIO::waba - SearchIO parser for Jim Kent WABA program


BioPerl documentation Contained in the BioPerl distribution.

Index


Code Index:

NAME

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Bio::SearchIO::waba - SearchIO parser for Jim Kent WABA program alignment output

SYNOPSIS

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    # do not use this object directly, rather through Bio::SearchIO

    use Bio::SearchIO;
    my $in = Bio::SearchIO->new(-format => 'waba',
			       -file   => 'output.wab');
    while( my $result = $in->next_result ) {
	while( my $hit = $result->next_hit ) {
	    while( my $hsp = $result->next_hsp ) {

	    }
	}
    }

DESCRIPTION

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This parser will process the waba output (NOT the human readable format).

FEEDBACK

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Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://redmine.open-bio.org/projects/bioperl/

AUTHOR - Jason Stajich

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Email jason-at-bioperl.org

APPENDIX

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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

 Title   : new
 Usage   : my $obj = Bio::SearchIO::waba->new();
 Function: Builds a new Bio::SearchIO::waba object 
 Returns : Bio::SearchIO::waba
 Args    : see Bio::SearchIO

next_result

 Title   : next_result
 Usage   : my $hit = $searchio->next_result;
 Function: Returns the next Result from a search
 Returns : Bio::Search::Result::ResultI object
 Args    : none

start_element

 Title   : start_element
 Usage   : $eventgenerator->start_element
 Function: Handles a start element event
 Returns : none
 Args    : hashref with at least 2 keys 'Data' and 'Name'




end_element

 Title   : start_element
 Usage   : $eventgenerator->end_element
 Function: Handles an end element event
 Returns : none
 Args    : hashref with at least 2 keys 'Data' and 'Name'




element

 Title   : element
 Usage   : $eventhandler->element({'Name' => $name, 'Data' => $str});
 Function: Convience method that calls start_element, characters, end_element
 Returns : none
 Args    : Hash ref with the keys 'Name' and 'Data'




characters

 Title   : characters
 Usage   : $eventgenerator->characters($str)
 Function: Send a character events
 Returns : none
 Args    : string




_mode

 Title   : _mode
 Usage   : $obj->_mode($newval)
 Function: 
 Example : 
 Returns : value of _mode
 Args    : newvalue (optional)




within_element

 Title   : within_element
 Usage   : if( $eventgenerator->within_element($element) ) {}
 Function: Test if we are within a particular element
           This is different than 'in' because within can be tested
           for a whole block.
 Returns : boolean
 Args    : string element name 




in_element

 Title   : in_element
 Usage   : if( $eventgenerator->in_element($element) ) {}
 Function: Test if we are in a particular element
           This is different than 'in' because within can be tested
           for a whole block.
 Returns : boolean
 Args    : string element name 




start_document

 Title   : start_document
 Usage   : $eventgenerator->start_document
 Function: Handles a start document event
 Returns : none
 Args    : none




end_document

 Title   : end_document
 Usage   : $eventgenerator->end_document
 Function: Handles an end document event
 Returns : Bio::Search::Result::ResultI object
 Args    : none




result_count

 Title   : result_count
 Usage   : my $count = $searchio->result_count
 Function: Returns the number of results we have processed
 Returns : integer
 Args    : none





BioPerl documentation Contained in the BioPerl distribution.
#
# BioPerl module for Bio::SearchIO::waba
#
# Please direct questions and support issues to <bioperl-l@bioperl.org> 
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself

# POD documentation - main docs before the code


# Let the code begin...


package Bio::SearchIO::waba;
use vars qw(%MODEMAP %MAPPING @STATES);
use strict;

# Object preamble - inherits from Bio::Root::Root

use Bio::Search::Result::ResultFactory;
use Bio::Search::HSP::HSPFactory;

use POSIX;

BEGIN { 
    # mapping of NCBI Blast terms to Bioperl hash keys
    %MODEMAP = ('WABAOutput' => 'result',
		'Hit'         => 'hit',
		'Hsp'         => 'hsp'
		);
    @STATES = qw(Hsp_qseq Hsp_hseq Hsp_stateseq);
    %MAPPING = 
	( 
	  'Hsp_query-from'=>  'HSP-query_start',
	  'Hsp_query-to'  =>  'HSP-query_end',
	  'Hsp_hit-from'  =>  'HSP-hit_start',
	  'Hsp_hit-to'    =>  'HSP-hit_end',
	  'Hsp_qseq'      =>  'HSP-query_seq',
	  'Hsp_hseq'      =>  'HSP-hit_seq',
	  'Hsp_midline'   =>  'HSP-homology_seq',
	  'Hsp_stateseq'  =>  'HSP-hmmstate_seq',
	  'Hsp_align-len' =>  'HSP-hsp_length',
	  
	  'Hit_id'        => 'HIT-name',
	  'Hit_accession' => 'HIT-accession',

	  'WABAOutput_program'  => 'RESULT-algorithm_name',
	  'WABAOutput_version'  => 'RESULT-algorithm_version',
	  'WABAOutput_query-def'=> 'RESULT-query_name',
	  'WABAOutput_query-db' => 'RESULT-query_database',
 	  'WABAOutput_db'       => 'RESULT-database_name',
	  );
}


use base qw(Bio::SearchIO);

sub _initialize {
    my ($self,@args) = @_;
    $self->SUPER::_initialize(@args);
    $self->_eventHandler->register_factory('result', Bio::Search::Result::ResultFactory->new(-type => 'Bio::Search::Result::WABAResult'));

    $self->_eventHandler->register_factory('hsp', Bio::Search::HSP::HSPFactory->new(-type => 'Bio::Search::HSP::WABAHSP'));
}


sub next_result{
    my ($self) = @_;
    local $/ = "\n";
    local $_;
    
    my ($curquery,$curhit);
    my $state = -1;
    $self->start_document();
    my @hit_signifs;
    while( defined ($_ = $self->_readline )) { 
	
	if( $state == -1 ) {
	    my ($qid, $qhspid,$qpercent, $junk,
		$alnlen,$qdb,$qacc,$qstart,$qend,$qstrand,
		$hitdb,$hacc,$hstart,$hend,
		$hstrand) =
		    ( /^(\S+)\.(\S+)\s+align\s+ # get the queryid
		      		      (\d+(\.\d+)?)\%\s+     # get the percentage
		      		      of\s+(\d+)\s+  # get the length of the alignment
		      		      (\S+)\s+           # this is the query database
		      		      (\S+):(\-?\d+)\-(\-?\d+) # The accession:start-end for query
		      		      \s+([\-\+])        # query strand
		      		      \s+(\S+)\.         # hit db
		      		      (\S+):(\-?\d+)\-(\-?\d+) # The accession:start-end for hit
		      		      \s+([\-\+])\s*$    # hit strand
		      		      /ox );
	    
	    # Curses.  Jim's code is 0 based, the following is to readjust
	    if( $hstart < 0 ) { $hstart *= -1}
	    if( $hend   < 0 ) { $hend   *= -1}
	    if( $qstart < 0 ) { $qstart *= -1}
	    if( $qend   < 0 ) { $qend   *= -1}
	    $hstart++; $hend++; $qstart++; $qend++;
	    if( ! defined $alnlen ) {
		$self->warn("Unable to parse the rest of the WABA alignment info for: '$_'");
		last;
	    }
	    $self->{'_reporttype'} = 'WABA'; # hardcoded - only 
	                                     # one type of WABA AFAIK	    
	    if( defined $curquery && 
		$curquery ne $qid ) { 
		$self->end_element({'Name' => 'Hit'});
		$self->_pushback($_);
		$self->end_element({'Name' => 'WABAOutput'});
		return $self->end_document();
	    } 
	    
	    if( defined $curhit &&
		$curhit ne $hacc) {
		# slight duplication here -- keep these in SYNC
		$self->end_element({'Name' => 'Hit'});
		$self->start_element({'Name' => 'Hit'});
		$self->element({'Name' => 'Hit_id',
				'Data' => $hacc});
		$self->element({'Name' => 'Hit_accession',
				'Data' => $hacc});

	    } elsif ( ! defined $curquery ) {
		$self->start_element({'Name' => 'WABAOutput'});
		$self->{'_result_count'}++;
		$self->element({'Name' => 'WABAOutput_query-def',
				'Data' => $qid });
		$self->element({'Name' => 'WABAOutput_program',
				'Data' => 'WABA'});
		$self->element({'Name' => 'WABAOutput_query-db',
				'Data' => $qdb});
		$self->element({'Name' => 'WABAOutput_db',
				'Data' => $hitdb});
		
		# slight duplication here -- keep these N'SYNC ;-)
		$self->start_element({'Name' => 'Hit'});
		$self->element({'Name' => 'Hit_id',
				'Data' => $hacc});
		$self->element({'Name' => 'Hit_accession',
				'Data' => $hacc});
	    }

	    
	    # strand is inferred by start,end values
	    # in the Result Builder
	    if( $qstrand eq '-' ) {
		($qstart,$qend) = ($qend,$qstart);
	    }
	    if( $hstrand eq '-' ) {
		($hstart,$hend) = ($hend,$hstart);
	    }

	    $self->start_element({'Name' => 'Hsp'});
	    $self->element({'Name' => 'Hsp_query-from',
			    'Data' => $qstart});
	    $self->element({'Name' => 'Hsp_query-to',
			    'Data' => $qend});
	    $self->element({'Name' => 'Hsp_hit-from',
			    'Data' => $hstart});
	    $self->element({'Name' => 'Hsp_hit-to',
			    'Data' => $hend});
	    $self->element({'Name' => 'Hsp_align-len',
			    'Data' => $alnlen});
	    
	    $curquery = $qid;
	    $curhit   = $hacc;
	    $state = 0;
	} elsif( ! defined $curquery ) {
	    $self->warn("skipping because no Hit begin line was recognized\n$_") if( $_ !~ /^\s+$/ );
	    next;
	} else { 
	    chomp;
	    $self->element({'Name' => $STATES[$state++],
			    'Data' => $_});
	    if( $state >= scalar @STATES ) {
		$state = -1;
		$self->end_element({'Name' => 'Hsp'});
	    }
	}
    }
    if( defined $curquery  ) {
	$self->end_element({'Name' => 'Hit'});
	$self->end_element({'Name' => 'WABAOutput'});
	return $self->end_document();
    }
    return;
}

sub start_element{
   my ($self,$data) = @_;
    # we currently don't care about attributes
    my $nm = $data->{'Name'};    
   if( my $type = $MODEMAP{$nm} ) {
	$self->_mode($type);
	if( $self->_eventHandler->will_handle($type) ) {
	    my $func = sprintf("start_%s",lc $type);
	    $self->_eventHandler->$func($data->{'Attributes'});
	}						 
	unshift @{$self->{'_elements'}}, $type;
    }
    if($nm eq 'WABAOutput') {
	$self->{'_values'} = {};
	$self->{'_result'}= undef;
	$self->{'_mode'} = '';
    }

}

sub end_element {
    my ($self,$data) = @_;
    my $nm = $data->{'Name'};
    my $rc;
    # Hsp are sort of weird, in that they end when another
    # object begins so have to detect this in end_element for now
    if( $nm eq 'Hsp' ) {
	foreach ( qw(Hsp_qseq Hsp_midline Hsp_hseq) ) {
	    $self->element({'Name' => $_,
			    'Data' => $self->{'_last_hspdata'}->{$_}});
	}
	$self->{'_last_hspdata'} = {}
    }

    if( my $type = $MODEMAP{$nm} ) {
	if( $self->_eventHandler->will_handle($type) ) {
	    my $func = sprintf("end_%s",lc $type);
	    $rc = $self->_eventHandler->$func($self->{'_reporttype'},
					      $self->{'_values'});	    
	}
	shift @{$self->{'_elements'}};

    } elsif( $MAPPING{$nm} ) { 	
	if ( ref($MAPPING{$nm}) =~ /hash/i ) {
	    my $key = (keys %{$MAPPING{$nm}})[0];	    
	    $self->{'_values'}->{$key}->{$MAPPING{$nm}->{$key}} = $self->{'_last_data'};
	} else {
	    $self->{'_values'}->{$MAPPING{$nm}} = $self->{'_last_data'};
	}
    } else { 
	$self->warn( "unknown nm $nm ignoring\n");
    }
    $self->{'_last_data'} = ''; # remove read data if we are at 
				# end of an element
    $self->{'_result'} = $rc if( $nm eq 'WABAOutput' );
    return $rc;

}

sub element{
   my ($self,$data) = @_;
   $self->start_element($data);
   $self->characters($data);
   $self->end_element($data);
}


sub characters{
   my ($self,$data) = @_;   

   return unless ( defined $data->{'Data'} );
   if( $data->{'Data'} =~ /^\s+$/ ) {
       return unless $data->{'Name'} =~ /Hsp\_(midline|qseq|hseq)/;
   }

   if( $self->in_element('hsp') && 
       $data->{'Name'} =~ /Hsp\_(qseq|hseq|midline)/ ) {
       
       $self->{'_last_hspdata'}->{$data->{'Name'}} .= $data->{'Data'};
   }  
   
   $self->{'_last_data'} = $data->{'Data'}; 
}

sub _mode{
    my ($self,$value) = @_;
    if( defined $value) {
	$self->{'_mode'} = $value;
    }
    return $self->{'_mode'};
}

sub within_element{
   my ($self,$name) = @_;  
   return 0 if ( ! defined $name &&
		 ! defined  $self->{'_elements'} ||
		 scalar @{$self->{'_elements'}} == 0) ;
   foreach (  @{$self->{'_elements'}} ) {
       if( $_ eq $name  ) {
	   return 1;
       } 
   }
   return 0;
}

sub in_element{
   my ($self,$name) = @_;  
   return 0 if ! defined $self->{'_elements'}->[0];
   return ( $self->{'_elements'}->[0] eq $name)
}


sub start_document{
    my ($self) = @_;
    $self->{'_lasttype'} = '';
    $self->{'_values'} = {};
    $self->{'_result'}= undef;
    $self->{'_mode'} = '';
    $self->{'_elements'} = [];
}


sub end_document{
   my ($self,@args) = @_;
   return $self->{'_result'};
}

sub result_count {
    my $self = shift;
    return $self->{'_result_count'};
}

sub report_count { shift->result_count }

1;