| BioPerl documentation | Contained in the BioPerl distribution. |
Bio::SearchIO::waba - SearchIO parser for Jim Kent WABA program alignment output
# do not use this object directly, rather through Bio::SearchIO
use Bio::SearchIO;
my $in = Bio::SearchIO->new(-format => 'waba',
-file => 'output.wab');
while( my $result = $in->next_result ) {
while( my $hit = $result->next_hit ) {
while( my $hsp = $result->next_hsp ) {
}
}
}
This parser will process the waba output (NOT the human readable format).
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://redmine.open-bio.org/projects/bioperl/
Email jason-at-bioperl.org
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new Usage : my $obj = Bio::SearchIO::waba->new(); Function: Builds a new Bio::SearchIO::waba object Returns : Bio::SearchIO::waba Args : see Bio::SearchIO
Title : next_result Usage : my $hit = $searchio->next_result; Function: Returns the next Result from a search Returns : Bio::Search::Result::ResultI object Args : none
Title : start_element Usage : $eventgenerator->start_element Function: Handles a start element event Returns : none Args : hashref with at least 2 keys 'Data' and 'Name'
Title : start_element Usage : $eventgenerator->end_element Function: Handles an end element event Returns : none Args : hashref with at least 2 keys 'Data' and 'Name'
Title : element
Usage : $eventhandler->element({'Name' => $name, 'Data' => $str});
Function: Convience method that calls start_element, characters, end_element
Returns : none
Args : Hash ref with the keys 'Name' and 'Data'
Title : characters Usage : $eventgenerator->characters($str) Function: Send a character events Returns : none Args : string
Title : _mode Usage : $obj->_mode($newval) Function: Example : Returns : value of _mode Args : newvalue (optional)
Title : within_element
Usage : if( $eventgenerator->within_element($element) ) {}
Function: Test if we are within a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name
Title : in_element
Usage : if( $eventgenerator->in_element($element) ) {}
Function: Test if we are in a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name
Title : start_document Usage : $eventgenerator->start_document Function: Handles a start document event Returns : none Args : none
Title : end_document Usage : $eventgenerator->end_document Function: Handles an end document event Returns : Bio::Search::Result::ResultI object Args : none
Title : result_count Usage : my $count = $searchio->result_count Function: Returns the number of results we have processed Returns : integer Args : none
| BioPerl documentation | Contained in the BioPerl distribution. |
# # BioPerl module for Bio::SearchIO::waba # # Please direct questions and support issues to <bioperl-l@bioperl.org> # # Cared for by Jason Stajich <jason@bioperl.org> # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code
# Let the code begin... package Bio::SearchIO::waba; use vars qw(%MODEMAP %MAPPING @STATES); use strict; # Object preamble - inherits from Bio::Root::Root use Bio::Search::Result::ResultFactory; use Bio::Search::HSP::HSPFactory; use POSIX; BEGIN { # mapping of NCBI Blast terms to Bioperl hash keys %MODEMAP = ('WABAOutput' => 'result', 'Hit' => 'hit', 'Hsp' => 'hsp' ); @STATES = qw(Hsp_qseq Hsp_hseq Hsp_stateseq); %MAPPING = ( 'Hsp_query-from'=> 'HSP-query_start', 'Hsp_query-to' => 'HSP-query_end', 'Hsp_hit-from' => 'HSP-hit_start', 'Hsp_hit-to' => 'HSP-hit_end', 'Hsp_qseq' => 'HSP-query_seq', 'Hsp_hseq' => 'HSP-hit_seq', 'Hsp_midline' => 'HSP-homology_seq', 'Hsp_stateseq' => 'HSP-hmmstate_seq', 'Hsp_align-len' => 'HSP-hsp_length', 'Hit_id' => 'HIT-name', 'Hit_accession' => 'HIT-accession', 'WABAOutput_program' => 'RESULT-algorithm_name', 'WABAOutput_version' => 'RESULT-algorithm_version', 'WABAOutput_query-def'=> 'RESULT-query_name', 'WABAOutput_query-db' => 'RESULT-query_database', 'WABAOutput_db' => 'RESULT-database_name', ); } use base qw(Bio::SearchIO);
sub _initialize { my ($self,@args) = @_; $self->SUPER::_initialize(@args); $self->_eventHandler->register_factory('result', Bio::Search::Result::ResultFactory->new(-type => 'Bio::Search::Result::WABAResult')); $self->_eventHandler->register_factory('hsp', Bio::Search::HSP::HSPFactory->new(-type => 'Bio::Search::HSP::WABAHSP')); }
sub next_result{ my ($self) = @_; local $/ = "\n"; local $_; my ($curquery,$curhit); my $state = -1; $self->start_document(); my @hit_signifs; while( defined ($_ = $self->_readline )) { if( $state == -1 ) { my ($qid, $qhspid,$qpercent, $junk, $alnlen,$qdb,$qacc,$qstart,$qend,$qstrand, $hitdb,$hacc,$hstart,$hend, $hstrand) = ( /^(\S+)\.(\S+)\s+align\s+ # get the queryid (\d+(\.\d+)?)\%\s+ # get the percentage of\s+(\d+)\s+ # get the length of the alignment (\S+)\s+ # this is the query database (\S+):(\-?\d+)\-(\-?\d+) # The accession:start-end for query \s+([\-\+]) # query strand \s+(\S+)\. # hit db (\S+):(\-?\d+)\-(\-?\d+) # The accession:start-end for hit \s+([\-\+])\s*$ # hit strand /ox ); # Curses. Jim's code is 0 based, the following is to readjust if( $hstart < 0 ) { $hstart *= -1} if( $hend < 0 ) { $hend *= -1} if( $qstart < 0 ) { $qstart *= -1} if( $qend < 0 ) { $qend *= -1} $hstart++; $hend++; $qstart++; $qend++; if( ! defined $alnlen ) { $self->warn("Unable to parse the rest of the WABA alignment info for: '$_'"); last; } $self->{'_reporttype'} = 'WABA'; # hardcoded - only # one type of WABA AFAIK if( defined $curquery && $curquery ne $qid ) { $self->end_element({'Name' => 'Hit'}); $self->_pushback($_); $self->end_element({'Name' => 'WABAOutput'}); return $self->end_document(); } if( defined $curhit && $curhit ne $hacc) { # slight duplication here -- keep these in SYNC $self->end_element({'Name' => 'Hit'}); $self->start_element({'Name' => 'Hit'}); $self->element({'Name' => 'Hit_id', 'Data' => $hacc}); $self->element({'Name' => 'Hit_accession', 'Data' => $hacc}); } elsif ( ! defined $curquery ) { $self->start_element({'Name' => 'WABAOutput'}); $self->{'_result_count'}++; $self->element({'Name' => 'WABAOutput_query-def', 'Data' => $qid }); $self->element({'Name' => 'WABAOutput_program', 'Data' => 'WABA'}); $self->element({'Name' => 'WABAOutput_query-db', 'Data' => $qdb}); $self->element({'Name' => 'WABAOutput_db', 'Data' => $hitdb}); # slight duplication here -- keep these N'SYNC ;-) $self->start_element({'Name' => 'Hit'}); $self->element({'Name' => 'Hit_id', 'Data' => $hacc}); $self->element({'Name' => 'Hit_accession', 'Data' => $hacc}); } # strand is inferred by start,end values # in the Result Builder if( $qstrand eq '-' ) { ($qstart,$qend) = ($qend,$qstart); } if( $hstrand eq '-' ) { ($hstart,$hend) = ($hend,$hstart); } $self->start_element({'Name' => 'Hsp'}); $self->element({'Name' => 'Hsp_query-from', 'Data' => $qstart}); $self->element({'Name' => 'Hsp_query-to', 'Data' => $qend}); $self->element({'Name' => 'Hsp_hit-from', 'Data' => $hstart}); $self->element({'Name' => 'Hsp_hit-to', 'Data' => $hend}); $self->element({'Name' => 'Hsp_align-len', 'Data' => $alnlen}); $curquery = $qid; $curhit = $hacc; $state = 0; } elsif( ! defined $curquery ) { $self->warn("skipping because no Hit begin line was recognized\n$_") if( $_ !~ /^\s+$/ ); next; } else { chomp; $self->element({'Name' => $STATES[$state++], 'Data' => $_}); if( $state >= scalar @STATES ) { $state = -1; $self->end_element({'Name' => 'Hsp'}); } } } if( defined $curquery ) { $self->end_element({'Name' => 'Hit'}); $self->end_element({'Name' => 'WABAOutput'}); return $self->end_document(); } return; }
sub start_element{ my ($self,$data) = @_; # we currently don't care about attributes my $nm = $data->{'Name'}; if( my $type = $MODEMAP{$nm} ) { $self->_mode($type); if( $self->_eventHandler->will_handle($type) ) { my $func = sprintf("start_%s",lc $type); $self->_eventHandler->$func($data->{'Attributes'}); } unshift @{$self->{'_elements'}}, $type; } if($nm eq 'WABAOutput') { $self->{'_values'} = {}; $self->{'_result'}= undef; $self->{'_mode'} = ''; } }
sub end_element { my ($self,$data) = @_; my $nm = $data->{'Name'}; my $rc; # Hsp are sort of weird, in that they end when another # object begins so have to detect this in end_element for now if( $nm eq 'Hsp' ) { foreach ( qw(Hsp_qseq Hsp_midline Hsp_hseq) ) { $self->element({'Name' => $_, 'Data' => $self->{'_last_hspdata'}->{$_}}); } $self->{'_last_hspdata'} = {} } if( my $type = $MODEMAP{$nm} ) { if( $self->_eventHandler->will_handle($type) ) { my $func = sprintf("end_%s",lc $type); $rc = $self->_eventHandler->$func($self->{'_reporttype'}, $self->{'_values'}); } shift @{$self->{'_elements'}}; } elsif( $MAPPING{$nm} ) { if ( ref($MAPPING{$nm}) =~ /hash/i ) { my $key = (keys %{$MAPPING{$nm}})[0]; $self->{'_values'}->{$key}->{$MAPPING{$nm}->{$key}} = $self->{'_last_data'}; } else { $self->{'_values'}->{$MAPPING{$nm}} = $self->{'_last_data'}; } } else { $self->warn( "unknown nm $nm ignoring\n"); } $self->{'_last_data'} = ''; # remove read data if we are at # end of an element $self->{'_result'} = $rc if( $nm eq 'WABAOutput' ); return $rc; }
sub element{ my ($self,$data) = @_; $self->start_element($data); $self->characters($data); $self->end_element($data); }
sub characters{ my ($self,$data) = @_; return unless ( defined $data->{'Data'} ); if( $data->{'Data'} =~ /^\s+$/ ) { return unless $data->{'Name'} =~ /Hsp\_(midline|qseq|hseq)/; } if( $self->in_element('hsp') && $data->{'Name'} =~ /Hsp\_(qseq|hseq|midline)/ ) { $self->{'_last_hspdata'}->{$data->{'Name'}} .= $data->{'Data'}; } $self->{'_last_data'} = $data->{'Data'}; }
sub _mode{ my ($self,$value) = @_; if( defined $value) { $self->{'_mode'} = $value; } return $self->{'_mode'}; }
sub within_element{ my ($self,$name) = @_; return 0 if ( ! defined $name && ! defined $self->{'_elements'} || scalar @{$self->{'_elements'}} == 0) ; foreach ( @{$self->{'_elements'}} ) { if( $_ eq $name ) { return 1; } } return 0; }
sub in_element{ my ($self,$name) = @_; return 0 if ! defined $self->{'_elements'}->[0]; return ( $self->{'_elements'}->[0] eq $name) }
sub start_document{ my ($self) = @_; $self->{'_lasttype'} = ''; $self->{'_values'} = {}; $self->{'_result'}= undef; $self->{'_mode'} = ''; $self->{'_elements'} = []; }
sub end_document{ my ($self,@args) = @_; return $self->{'_result'}; }
sub result_count { my $self = shift; return $self->{'_result_count'}; } sub report_count { shift->result_count } 1;