Bio::SeqIO::pir - PIR sequence input/output stream

BioPerl documentation Contained in the BioPerl distribution.

Index

Code Index:

NAME

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Bio::SeqIO::pir - PIR sequence input/output stream

SYNOPSIS

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Do not use this module directly. Use it via the Bio::SeqIO class.

DESCRIPTION

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This object can transform Bio::Seq objects to and from pir flat file databases.

Note: This does not completely preserve the PIR format - quality information about sequence is currently discarded since bioperl does not have a mechanism for handling these encodings in sequence data.

FEEDBACK

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Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

  https://redmine.open-bio.org/projects/bioperl/

AUTHORS

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Aaron Mackey <amackey@virginia.edu> Lincoln Stein <lstein@cshl.org> Jason Stajich <jason@bioperl.org>

APPENDIX

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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

next_seq

 Title   : next_seq
 Usage   : $seq = $stream->next_seq()
 Function: returns the next sequence in the stream
 Returns : Bio::Seq object
 Args    : NONE

write_seq

 Title   : write_seq
 Usage   : $stream->write_seq(@seq)
 Function: writes the $seq object into the stream
 Returns : 1 for success and 0 for error
 Args    : Array of Bio::PrimarySeqI objects
BioPerl documentation Contained in the BioPerl distribution. 
#
# BioPerl module for Bio::SeqIO::PIR
#
# Please direct questions and support issues to <bioperl-l@bioperl.org> 
#
# Cared for by Aaron Mackey <amackey@virginia.edu>
#
# Copyright Aaron Mackey
#
# You may distribute this module under the same terms as perl itself
#
# _history
# October 18, 1999  Largely rewritten by Lincoln Stein

# POD documentation - main docs before the code


# Let the code begin...

package Bio::SeqIO::pir;
use strict;

use Bio::Seq::SeqFactory;

use base qw(Bio::SeqIO);

our %VALID_TYPE = map {$_ => 1} qw(P1 F1 DL DC RL RC XX);

sub _initialize {
  my($self,@args) = @_;
  $self->SUPER::_initialize(@args);
  if( ! defined $self->sequence_factory ) {
      $self->sequence_factory(Bio::Seq::SeqFactory->new
			      (-verbose => $self->verbose(),
			       -type => 'Bio::Seq'));
  }
}


sub next_seq {
    my ($self) = @_;
    local $/ = "\n>";
    return unless my $line = $self->_readline;
    if( $line eq '>' ) {	# handle the very first one having no comment
	return unless $line = $self->_readline;
    }
    my ($top, $desc,$seq) = ( $line =~ /^(.+?)\n(.+?)\n([^>]*)/s )  or
	$self->throw("Cannot parse entry PIR entry [$line]");

    my ( $type,$id );
	if ( $top =~ /^>?(\S{2});(\S+)\s*$/ ) {
	  ( $type,$id ) = ($1, $2);
	  if (!exists $VALID_TYPE{$type} ) {
		$self->throw("PIR stream read attempted without proper two-letter sequence code [ $type ]");
	  }
	} else {
	  $self->throw("Line does not match PIR format [ $line ]");
	}

    # P - indicates complete protein
    # F - indicates protein fragment
    # not sure how to stuff these into a Bio object
    # suitable for writing out.
    $seq =~ s/\*//g;
    $seq =~ s/[\(\)\.\/\=\,]//g;
    $seq =~ s/\s+//g;		# get rid of whitespace

    my ($alphabet) = ('protein');
    # TODO - not processing SFS data
    return $self->sequence_factory->create
	(-seq        => $seq,
	 -primary_id => $id,
	 -id         => $id,
	 -desc       => $desc,
	 -alphabet   => $alphabet
	 );
}


sub write_seq {
    my ($self, @seq) = @_;
    for my $seq (@seq) {
	$self->throw("Did not provide a valid Bio::PrimarySeqI object")
	    unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI');

        $self->warn("No whitespace allowed in PIR ID [". $seq->display_id. "]")
            if $seq->display_id =~ /\s/;

	my $str = $seq->seq();
	return unless $self->_print(">P1;".$seq->id(),
				    "\n", $seq->desc(), "\n",
				    $str, "*\n");
    }

    $self->flush if $self->_flush_on_write && defined $self->_fh;
    return 1;
}

1;