Bio::Tools::FootPrinter - write sequence features in FootPrinter format


BioPerl documentation Contained in the BioPerl distribution.

Index


Code Index:

NAME

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Bio::Tools::FootPrinter - write sequence features in FootPrinter format

SYNOPSIS

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    use Bio::Tools::FootPrinter;

    my $tool = Bio::Tools::FootPrinter->new(-file=>"footprinter.out");

    while (my $result = $tool->next_feature){
      foreach my $feat($result->sub_SeqFeature){
        print $result->seq_id."\t".$feat->start."\t".$feat->end."\t".$feat->seq->seq."\n";
      }
    }

DESCRIPTION

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This module writes sequence features in FootPrinter format. See http://bio.cs.washington.edu/software.html for more details.

FEEDBACK

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Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://redmine.open-bio.org/projects/bioperl/

AUTHOR - Shawn Hoon

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Email shawnh@fugu-sg.org

APPENDIX

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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

 Title   : new
 Usage   : my $obj = Bio::Tools::FootPrinter->new();
 Function: Builds a new Bio::Tools::FootPrinter object 
 Returns : Bio::Tools::FootPrinter
 Args    : -fh/-file => $val, # for initing input, see Bio::Root::IO

next_feature

 Title   : next_feature
 Usage   : my $r = $footprint->next_feature
 Function: Get the next feature from parser data
 Returns : L<Bio::SeqFeature::Generic> 
 Args    : none

_add_feature

 Title   : _add_feature
 Usage   : $footprint->_add_feature($feat)
 Function: Add feature to array
 Returns : none
 Args    : none

_parse_predictions

 Title   : _parse_predictions
 Usage   : my $r = $footprint->_parse_predictions
 Function: do the parsing 
 Returns : none 
 Args    : none

_predictions_parsed

 Title   : _predictions_parsed
 Usage   : $footprint->_predictions_parsed(1)
 Function: Get/Set for whether predictions parsed
 Returns : 1/0
 Args    : none

_parse

 Title   : _parse
 Usage   : $footprint->_parse($name,$seq,$pattern)
 Function: do the actual parsing
 Returns : L<Bio::SeqFeature::Generic>
 Args    : none


BioPerl documentation Contained in the BioPerl distribution.
# BioPerl module for Bio::Tools::FootPrinter
#
# Please direct questions and support issues to <bioperl-l@bioperl.org> 
#
# Cared for by Shawn Hoon <shawnh@fugu-sg.org>
#
# Copyright Shawn Hoon
#
# You may distribute this module under the same terms as perl itself

# POD documentation - main docs before the code


# Let the code begin...


package Bio::Tools::FootPrinter;
use strict;

use Bio::SeqFeature::Generic;
use Bio::PrimarySeq;

use base qw(Bio::Root::Root Bio::Root::IO);

sub new {
  my($class,@args) = @_;

  my $self = $class->SUPER::new(@args);
  $self->_initialize_io(@args);

  return $self;
}

sub next_feature{
   my ($self) = @_;
   $self->_parse_predictions() unless $self->_predictions_parsed();
   return shift @{$self->{'_feature'}};

}

sub _add_feature {
    my ($self,$feat) = @_;
    if($feat){
        push @{$self->{'_feature'}},$feat;
    }
}

sub _parse_predictions {
  my ($self) = @_;
  $/="";
  my ($seq,$second,$third,$name);
  while ($_ = $self->_readline) {
    chomp;
    my @array = split("\n",$_);
    if ($#array == 5) {
      # get rid of header
      shift(@array); shift(@array);
    }
    if($#array == 3){
        if($name){
            $name=~s/>//;
            my $feat = $self->_parse($name,$seq,$second,$third);
            $self->_add_feature($feat);
        }
        $name    = shift @array;
        $seq     = $array[0];
        $second  = $array[1];
        $third   = $array[2];
        next;
    }
    $seq        .= $array[0];
    $third      .= $array[2];
  }
  
  $seq || return;
  
  $name=~s/>//;
  my $feat = $self->_parse($name,$seq,$second,$third);
  $self->_add_feature($feat);

  $self->_predictions_parsed(1);
}

sub _predictions_parsed {
    my ($self,$val) = @_;
    if($val){
        $self->{'_predictions_parsed'} = $val;
    }
    return $self->{'_predictions_parsed'};
}


sub _parse {
    my ($self,$name,$seq,$score,$pattern) = @_;
    my @char  = split('',$pattern);
    my @score = split('',$score);

    my ($prev,$word,@words,@word_scores,$word_score);

    my $i = 0;
    for my $c ( @char ) {
        if( ! $word) {
            $word .= $c;
            $prev = $c;
	    defined $score[$i] && 
		($score[$i] =~ /\d/) && ($word_score += $score[$i]);
        } elsif ($c eq $prev){
	    $word .=$c;
	    $prev  = $c;
	    defined $score[$i] && 
		($score[$i] =~ /\d/) && ($word_score += $score[$i]);
        } else {
            # remove words with only \s
            $word=~s/\s+//g;
            if ($word ne ''){
		push @words, $word;
		push @word_scores, ($word_score/length($word));
            }
            $word =$c;
	    $prev = $c;
	    $word_score = 0;
	    defined $score[$i] &&
		($score[$i] =~ /\d/) && ($word_score += $score[$i]);
        }
	$i++;
    }
    $word =~s/\s+//g;
    if( length($word) ){
	push @words, $word;
    }
    my $last;
    my $feat = Bio::SeqFeature::Generic->new(-seq_id=>$name);
    my $offset = $i = 0;
    my $count = 1;
    for my $w (@words){
        if(length($w) ) { 
	    my $index = index($pattern,$w,$offset);
	    $offset = $index + length($w);
	    my $subfeat = Bio::SeqFeature::Generic->new 
		( -seq_id  =>"$name-motif".$count++,
		  -start   => $index+1, 
		  -end     => $index+length($w),
		  -source  =>"FootPrinter",
		  -score   => $word_scores[$i]
		  );
	    # ugh - not sure the sub_SeqFeature situation will
	    # be around forever- things should probably be
	    # grouped by a 'group' tag instead ala GFF3 
	    # perhaps when Lincoln's API changes are 
	    # made to SeqFeatures this will get changed
	    $feat->add_sub_SeqFeature($subfeat,'EXPAND');
        }
	$i++;
    }
    my $priseq = Bio::PrimarySeq->new(-id=>$name,-seq=>$seq);
    $feat->attach_seq($priseq);
    return $feat;

}

1;